illumina MiniSeq User Manual

imaging compartment 2
imaging, 2-channel sequencing 50
indexing considerations 51
instrument
configuration settings 45
power button 4
start up 8
instrument maintenance
consumables 14
instrument wash 32
intensities 50
InterOp files 38, 52
L
laboratory-grade water guidelines 14
Local Run Manager 4
locs files 52
log files
GlobalLog 40
LaneNLog 40
M
maintenance, preventive 32
manage instrument
shut down 46
metrics
base calling 50
cluster density cycles 29
intensity cycles 29
miRbase database 7
O
output files 52
output files, sequencing 52
P
passing filter (PF) 50
phasing, prephasing 49
Phred algorithm 51
post-run wash 30
power button 4, 8
power switch 8
pre-run check 24, 28
pre-run check errors 39
preventive maintenance 32
primer rehybridization 41
purge consumables 9
Q
Q-scores 51
quality tables 51
R
read length 16
reagent cartridge
overview 6
preparation 19
reservoir #28 33
reagent compartment 2
reagents
kitted 5
proper disposal 22, 26
Real-Time Analysis software 1, 4
results 52
reference genomes
custom genomes 46
file format 7
preinstalled 7
RefGene database 7
rehybridization, Read 1 41
RFID tracking 5
RTA v2
overview 48
termination 48
RTA2
error handling 40
run copy service 29
run duration 16
run metrics 28
run parameters
BaseSpace mode 24
edit parameters 24
standalone mode 23
run setup, advanced option 9
RunInfo.xml 38, 52
S
Sequencing Analysis Viewer 16
sequencing workflow 49
shutting down the instrument 46
sodium hypochlorite, wash 33
software
analysis 4
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
55
MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH