illumina NextSeq 500 User Manual

Chapter 1 Overview
Introduction 1
Additional Resources 1
Instrument Components 2
Sequencing Consumables Overview 4
Introduction
The Illumina
®
NextSeq
™
500 system combines the power of high-throughput sequencing with the simplicity
of a desktop sequencing instrument.
Features
u High-throughput sequencing—The NextSeq 500 enables sequencing of exomes, whole genomes, and
transcriptomes and supports TruSeq
®
and Nextera
®
libraries.
u Flow cell types—Flow cells are available in configurations for high output and mid output. Each flow cell
type is kitted with a compatible prefilled reagent cartridge.
u Real-Time Analysis (RTA)—Integrated analysis software performs on-instrument data analysis, which
includes image analysis and base calling. The NextSeq uses an implementation of RTA called RTA v2,
which includes important architecture and feature differences. For more information, see Real-Time
Analysis on page 38.
u BaseSpace
®
integration—The sequencing workflow is integrated with BaseSpace, the Illumina genomics
computing environment for data analysis, storage, and collaboration. For instruments configured for
BaseSpace, library information and run parameters are specified on the BaseSpace Prep tab. Runs that
were set up in BaseSpace appear on the instrument interface during run setup. As the run progresses,
output files are streamed in real time to BaseSpace or BaseSpace Onsite.
Additional Resources
The following documentation is available for download from the Illumina website.
Resource Description
NextSeq System Site Prep Guide
(document # 15045113)
Provides specifications for laboratory space, electrical requirements, and
environmental considerations.
NextSeq System Safety and
Compliance Guide (document #
15046564)
Provides information about operational safety considerations, compliance
statements, and instrument labeling.
RFID Reader - Model # TR-001-44
User Guide (document # 15041950)
Provides information about the RFID reader in the instrument, compliance
certifications, and safety considerations.
Denaturing and Diluting Libraries for
the NextSeq System (document #
15048776)
Provides instructions for denaturing and diluting prepared libraries for a
sequencing run, and preparing an optional PhiX control. This step applies to
most library types.
NextSeq Custom Primers Guide
(document # 15057456)
Provides information about using custom sequencing primers in place of
Illumina sequencing primers.
BaseSpace help
(help.basespace.illumina.com)
Provides information about using BaseSpace
®
and available analysis options.
Visit the NextSeq 500 support page on the Illumina website for access to documentation, software
downloads, online training, and frequently asked questions.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
1

illumina NextSeq 500 Specifications

General

Brand	illumina
Model	NextSeq 500
Category	Laboratory Equipment
Language	English