Document Date Description of Change
15046563 B February
2014
Updated software descriptions to NextSeq Control Software v1.1:
• Added search feature on Run Setup screen to filter list of available
runs.
• Added that available recipes include NextSeq High or NextSeq Mid,
depending on flow cell type.
• Noted that software updates include license agreement, release
notes, and list of software to be updated.
• Added description of the RAID error message.
• Noted that an Exit button closes NSSand restarts NCS automatically
at end of a system check.
Added reagent storage duration of up to 1 week at 4°C.
Updated reagent cartridge label for reservoir #10 to Load Library Here.
Updated user-supplied consumables list to specify 3%–6% sodium
hypochlorite and list a supplier catalog number.
Updated instructions for preparing a 0.06% dilution of NaOCl for
instrument washes, which includes a reduction in volume to 2 ml and a
3%–6% starting concentration.
Added illustrations to show good and bad clip position on the flow cell.
Updated the Real-Time Analysis chapter to include an overview of RTA
v2, the output folder structure, and the base calling process.
Updated troubleshooting chapter to include RTA v2 errors and include
RunParameters.xml in the list of troubleshooting files.
15046563 A January
2014
Initial release.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
vi
NextSeq 500 System Guide
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