Document Date Description of Change
15046563 D June 2014 Added instructions to load BP13 in position #18 of the reagent cartridge
when performing dual-indexed runs.
Corrected cycle at which cluster density metrics appear, which is cycle
25.
Corrected reagent cartridge positions for custom primers as position #7
(Read 1), #8 (Read 2), and #9 (Index 1).
Added a note about potential damage from relocating the instrument
after installation. Always contact your Illumina representative before
relocating the instrument.
Updated URL for Safety Data Sheets (SDS) to
support.illumina.com/sds.ilmn.
15046563 C April 2014 Updated software descriptions to NextSeq Control Software v1.2 and
RTA v2.1:
• Added recipe name NextSeq Mid for use with the NextSeq 500 Mid
Output Kit.
• Removed instructions to add NaOCl to the buffer wash cartridge for a
manual wash.
• Corrected NaOCl volume to 3 ml for position 28 of the reagent
cartridge, which is required for the automatic post-run wash.
• Noted that long run names appear in a scrolling field on the Run
Setup screen.
• Noted that RTA v2 does not apply phasing and prephasing corrections
to index reads.
• Added description of log files to list of files used for troubleshooting.
• Added instructions for emptying a full spent reagents container during
a run.
• Added description of recipe folders, including location of custom
recipes.
• Added description of thermal checks for fans and thermal probes.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
v
NextSeq 500 System Guide
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