Document Date Description of Change
15046563 G February
2015
Updated software descriptions to NextSeq Control Software v1.4.
• Added manual wash options: Quick Wash and Manual Post-Run
Wash.
• Added description of run customization features to purge
consumables at end of run and to skip pre-run check confirmation.
• Added option to cancel and restart a pre-run check.
• Added option to enable run monitoring in standalone mode.
• Removed description of offsets files and phasing files, which are no
longer written to the run folder.
• Updated scatter plot image to show more evenly distributed base calls
when using version 1.4 of the system software.
• Added description of Run Copy Service.
• Added option to use a custom Index 2 primer, possible with the
NextSeq 500 Kit v2. For more information, see
NextSeq Custom
Primers Guide (document # 15057456)
.
Updated reagent prep instructions for using the NextSeq 500 Kit v2:
Removed manual steps for loading sodium hypochlorite and dual-
indexing primers to the reagent cartridge. These reagents are prefilled in
the v2 reagent cartridge. For more information, see the
NextSeq
500/550 Kit v2 Reference Guide (document # 15058065)
.
Added Sequencing Consumables section that lists kit versions,
compatible NCS versions, and the name and part number of the
associated kit reference guide.
Updated user-supplied consumables to specify uses of NaOCl for
manual wash options introduced in NCS v1.4.
Corrected the requirements for clusters passing filter to no more than 1
base call below chastity in the first 25 cycles.
Added volume of 120 ml of laboratory-grade water to the System Check
instructions.
15046563 F September
2014
Corrected feature descriptions for NextSeq Control Software v1.3.
Updated URL for Safety Data Sheets (SDS) to
support.illumina.com/sds.html.
Updated NextSeq product markings from ™ to ®.
15046563 E August
2014
Updated software descriptions to NextSeq Control Software v1.3:
• Updated descriptions of the System Customization and Software
Updates commands on the Manage Instrument screen.
• Updated description of the Pre-Run Check screen, which groups the
checked items into 4 expandable categories.
• Updated the Live Help instructions to access Live Help using the URL.
The icon on the Home screen is not available in NCS v1.3.
• Added the on-instrument rehybridization procedure to rehybridize the
Read 1 primer. The option to rehybridize a flow cell is compatible with
NCS v1.3, or later, and requires a new reagent cartridge and buffer
cartridge.
Added
NextSeq Custom Primers Guide (document # 15057456)
to list
of additional resources.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
iv
NextSeq 500 System Guide
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