Revision History
Document Date Description of Change
Material # 20006818
Document # 15046563
v04
May 2018
Added support for NextSeq v2.5 reagents.
Updated storage/shipping information for NextSeq v2.5 Reagent Kits
shipping flow cells at ambient temperatures. NextSeq v2.5 flow cells
continue to require previous storage conditions.
Added information about NextSeq v2.5 Reagent Kits requiring software
updates to version 2.2.
Added note regarding mid output kit loading concentration.
Added note regarding saving flow cells.
Added note recommending that high output flow cells are used for
system checks.
Material # 20006818
Document # 15046563
v03
March
2018
Removed the default user name and password required to log on to the
operating system. Illumina recommends using site-specific credentials.
Added information about the Illumina Proactive monitoring service in the
Select BaseSpace Configuration section.
Updated RTA v2 software references to RTA2.
Material # 20006818
Document # 15046563
v02
March
2016
Added section titled Indexing Considerations.
Removed steps to inspect the flow cell.
Specified loading volume and concentration in the step to Load
Libraries onto the Reagent Cartridge.
Material # 20001879
Document # 15046563
v01
October
2015
Specified that an equivalent to the recommended supplier of NaOCl is a
laboratory-grade equivalent.
Added recommendation for annual preventive maintenance service.
Reorganized information in the Overview and Getting Started chapters.
Added instructions for customizing system settings.
Removed Live Help instructions from troubleshooting chapter. This
feature was removed from the control software.
15046563 I May 2015 Corrected description of reserved reservoirs on the reagent cartridge.
15046563 H May 2015 Corrected the volume of Tween 20 in the wash solution used for
manual washes.
Restructured information about system configuration options.
Moved information about Real-Time Analysis software to Appendix A.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
iii
NextSeq 500 System Guide
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