Figure 2 Reagent and Buffer Compartments
A Reagent compartment door—Encloses the reagent compartment with a latch under the lower-right corner
of the door. The reagent compartment holds the reagent cartridge. Reagents are pumped through the
sippers and fluidics system, and then to the flow cell.
B Reagent cartridge—The reagent cartridge is a prefilled single-use consumable.
C Buffer cartridge—The buffer cartridge is a prefilled single-use consumable.
D Spent reagents container—Spent reagents are collected for disposal after each run.
E Buffer compartment door—Encloses the buffer compartment with a latch under the lower-left corner of the
door.
NextSeq Software
The instrument software includes integrated applications that perform sequencing runs.
u NextSeq Control Software (NCS)—The control software guides you through the steps to set up a
sequencing run.
u Real-Time Analysis (RTA) software—RTA performs image analysis and base calling during the run. The
NextSeq 500 uses RTA v2, which includes important architecture and feature differences from earlier
versions. For more information, see
Real-Time Analysis
on page 38.
Status Icons
A status icon in the top-right corner of the control software interface screen signals any change in conditions
during run setup or during the run.
Status
Icon
Status Name Description
Status OK System is normal.
Processing System is processing.
Warning A warning has occurred.
Warnings do not stop a run or require action before proceeding.
Error An error has occurred.
Errors require action before proceeding with the run.
When a change in condition occurs, the icon blinks to alert you. Select the icon to view a description of the
condition. Select Acknowledge to accept the message and Close to close the dialog box.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
3
NextSeq 500 System Guide
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