Camera Numbering
The NextSeq 500 uses 6 cameras to image the flow cell.
Cameras are numbered 1–6. Cameras 1–3 image lane 1. Cameras 4–6 image lane 3. After lanes 1 and 3 are
imaged, the imaging module moves on the X-axis to image lanes 2 and 4.
Figure 26 Camera and Segment Numbering (High output flow cell shown)
Tile Numbering
There are 12 tiles in each swath of each camera segment. Tiles are numbered 01–12, regardless of swath
number or camera segment, and represented in 2 digits.
Figure 27 Tile Numbering
The complete tile number includes 5 digits to represent the location, as follows:
u Surface—1 represents the top surface; 2 represents the bottom surface
u Swath—1, 2, or 3
u Camera—1, 2, 3, 4, 5, or 6
u Tile—01, 02, 03, 04, 05, 06, 07, 08, 09, 10, 11, or 12
Example: Tile number 12508 indicates top surface, swath 2, camera 5, and tile 8.
The complete 5-digit tile number is used in the file name of thumbnail images and empirical phasing files. For
more information, see
Sequencing Output Files
on page 42.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
44
NextSeq 500 System Guide
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