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illumina NextSeq 550Dx User Manual

illumina NextSeq 550Dx
75 pages
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Table of Contents
Chapter 1 Overview 1
About this Guide 1
Introduction 1
Additional Resources 2
Instrument Components 2
Sequencing Consumables Overview 6
Chapter 2 Getting Started 11
Starting the Instrument 11
Customize System Settings 12
User-Supplied Consumables and Equipment 13
Chapter 3 Sequencing 15
Introduction 15
Sequencing Workflow 16
Prepare the Reagent Cartridge 16
Prepare the Flow Cell 17
Prepare Libraries for Sequencing 17
Set Up a Sequencing Run 18
Monitor Run Progress 24
Automatic Post-Run Wash 26
Chapter 4 Scanning 27
Introduction 27
Scanning Workflow 28
Download the DMAP Folder 28
Load the BeadChip Onto the Adapter 29
Set Up a Scan 30
Monitor Scan Progress 32
Chapter 5 Maintenance 35
Introduction 35
Perform a Manual Wash 35
Replace Air Filter 38
Software Updates 39
Reboot and Shut Down Options 40
Appendix A Troubleshooting 43
Introduction 43
Troubleshooting Files 43
Resolve Automatic Check Errors 44
Spent Reagents Container is Full 45
Rehybridization Workflow 46
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
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illumina NextSeq 550Dx Specifications

General IconGeneral
Brandillumina
ModelNextSeq 550Dx
CategoryLaboratory Equipment
LanguageEnglish

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