Imaging System Recommended Action
Imaging Limits Contact Illumina Technical Support.
Z Steps-and-Settle Contact Illumina Technical Support.
Bit Error Rate Contact Illumina Technical Support.
Flow Cell
Registration
It is possible that the flow cell is not properly seated.
• On the run setup screens, select Bac k to return to the flow cell step. The imaging
compartment door opens.
• Unload and reload the flow cell to make sure that it is seated properly.
Reagent Delivery Recommended Action
Valve Response Contact Illumina Technical Support.
Pump Contact Illumina Technical Support.
Buffer Mechanism Contact Illumina Technical Support.
Spent Reagents
Empty
Empty the spent reagents container and reload the empty container.
Checks for Array Scans
System Checks Recommended Action
Doors Closed Make sure that the compartment doors are closed.
Consumables Loaded Consumable sensors do not register. Make sure that each consumable is properly
loaded.
On the run setup screens, select Bac k to return to the loading step, and repeat run
setup.
Required Software Critical components of the software are missing.
Perform a manual software update to restore all software components.
Verify Input Files Make sure that the path to the cluster file and manifest file is correct and the files are
present.
Instrument Disk Space The instrument hard drive does not have sufficient disk space to perform a run. It is
possible that data from a previous run did not transfer.
Clear run data from the instrument hard drive.
Network Connection The network connection has been interrupted. Check network status and the physical
network connection.
Network Disk Space Either the BaseSpace account is full or the network server is full.
Imaging System Recommended Action
Imaging Limits Contact Illumina Technical Support.
Z Steps-and-Settle Contact Illumina Technical Support.
Bit Error Rate Contact Illumina Technical Support.
Auto-Center Unload the BeadChip adapter. Make sure that the BeadChip is seated in the adapter,
and then reload the adapter.
Spent Reagents Container is Full
Always begin a run with an empty spent reagents container.
If you begin a run without emptying the spent reagents container, system sensors trigger the software to
pause the run when the container is full. System sensors cannot pause a run during clustering, paired-
end resynthesis, or the automatic post-run wash.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
45
NextSeq 550Dx Research Mode Instrument Reference Guide
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