illumina NextSeq 550Dx User Manual

u Upon instrument initialization
u When a run is started
u When certain errors occur
u When user interaction is required
u When a run has finished
4 Select Save to save settings and exit the screen.
Set Run Setup Options
1 From the Manage Instrument screen, select System Customization.
2 Select the Use Advanced Load Consumables checkbox to enable the option to load all run
consumables from a single screen.
3 Select the Skip Pre-Run Check Confirmation checkbox to start sequencing or scanning
automatically after a successful automatic check.
4 Select Save to save settings and exit the screen.
Set Automatic Purge Option
1 From the Manage Instrument screen, select System Customization.
2 Select the Purge Consumables at End of Run checkbox to purge unused reagents from the
reagent cartridge to the spent reagents container automatically after each run.
NOTE
Purging consumables automatically adds additional time to the workflow.
3 Select Save to save settings and exit the screen.
User-Supplied Consumables and Equipment
The following consumables and equipment are used for consumables preparation, sequencing, and
instrument maintenance.
User-Supplied Consumables for Sequencing Runs
Consumable Supplier Purpose
1 N NaOH
(sodium hydroxide)
General lab supplier Library denaturation, diluted to 0.2N
200 mM Tris-HCl, pH7 General lab supplier Library denaturation
Alcohol wipes, 70% Isopropyl
or
Ethanol, 70%
VWR, catalog#95041-714
(or equivalent)
General lab supplier
Flow cell cleaning and general purpose
Lab tissue, low-lint VWR, catalog#21905-026
(or equivalent)
Flow cell cleaning and general purpose
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
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NextSeq 550Dx Research Mode Instrument Reference Guide

illumina NextSeq 550Dx Specifications

General

Brand	illumina
Model	NextSeq 550Dx
Category	Laboratory Equipment
Language	English