Several reservoirs are reserved for the automatic post-run wash. Wash solution is pumped from the
buffer cartridge to the reserved reservoirs, through the system, and then to the spent reagents container.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur
through inhalation, ingestion, skin contact, and eye contact. Wear protective equipment,
including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle
used reagents as chemical waste and discard in accordance with applicable regional, national,
and local laws and regulations. For additional environmental, health, and safety information, see the
SDS at support.illumina.com/sds.html.
Reserved Reservoirs
Figure 6 Numbered Reservoirs
Position Description
7, 8, and 9 Reserved for optional custom primers
10 Load libraries
For information about custom primers, see
NextSeq Custom Primers Guide (document # 15057456)
.
Removable Reservoir in Position #6
The prefilled reagent cartridge includes a denaturation reagent in position 6 that contains formamide. To
facilitate safe disposal of any unused reagent after the sequencing run, the reservoir in position 6 is
removable. For more information, see
Remove Used Reservoir from Position #6
on page 22.
Buffer Cartridge Overview
The buffer cartridge is a single-use consumable containing three reservoirs that are prefilled with buffers
and wash solution. The contents of the buffer cartridge are sufficient for sequencing one flow cell.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
8
NextSeq 550Dx Research Mode Instrument Reference Guide
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