Required Consumables
A rehybridization run requires a new NextSeq 550Dx reagent cartridge and buffer cartridge regardless of
when the run was stopped.
End the Current Run
1 Select End Run. When prompted to confirm the command, select Yes.
2 When prompted to save the flow cell, select Yes. Note the expiration date for rehybridization.
3 Remove the saved flow cell and set aside at 2°C to 8°C until you are ready to set up the
rehybridization run.
NOTE
You can store the flow cell up to 7 days at 2°C to 8°C in the plastic clamshell case
without
the
desiccant package. For best results, rehybridize the saved flow cell within 3 days.
Perform a Manual Wash
1 From the Home screen, select Perform Wash.
2 From the Wash Selection screen, select Manual Post-Run Wash. See
Perform a Manual Wash
on
page 35.
NOTE
If you have not removed the reagent cartridge and buffer cartridge from the stopped run, you can use
them for the manual wash. Otherwise, perform the manual wash with the reagent wash cartridge and
buffer wash cartridge.
Set Up a New Run on the BaseSpace Prep Tab
1 If the instrument is configured for BaseSpace or BaseSpace Onsite, set up a new run on the Prep
tab using the same parameters as the original run.
TIP
Click the Pools tab, select the appropriate Pool ID to retain the previous run settings, and then assign
a unique name for the new run.
Set Up a Run on the Instrument
1 Prepare a new reagent cartridge.
2 If the saved flow cell was stored, allow it to reach room temperature (15–30 minutes).
3 Clean and load the saved flow cell.
4 Remove the spent reagents container and discard the contents appropriately, and then reload the
empty container.
5 Load the new buffer cartridge and reagent cartridge.
6 From the Run Setup screen, select from the following options:
u BaseSpace or BaseSpace Onsite—Select the run and confirm run parameters.
u Standalone—Enter the name of the run and specify the same parameters as the original run.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
47
NextSeq 550Dx Research Mode Instrument Reference Guide
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