illumina NextSeq 550Dx User Manual

2 Select Next.
3 Confirm run parameters.
u Run Name—Name of the run as assigned in BaseSpace.
u Library ID—Name of the pooled libraries as assigned in BaseSpace.
u Recipe—Name of the recipe, either NextSeq High or NextSeq Mid depending on the reagent
cartridge used for the run.
u Read Type—Single Read or Paired End.
u Read Length—Number of cycles for each read.
u [Optional] Custom Primers, if applicable.
4 [Optional] Select the Edit button to change run parameters. When finished, select Save.
u Run parameters—Change the number of reads or number of cycles per read.
u Custom primers—Change the settings for custom primers. For more information, see NextSeq
Custom Primers Guide (document # 15057456) .
u Purge consumables for this run—Change the setting to purge consumables automatically after
the current run.
5 Select Next.
Enter Run Parameters (Standalone Configuration)
1 Enter a run name of your preference.
2 [Optional] Enter a library ID of your preference.
3 Select a read type, either Single Read or Paired End.
4 Enter the number of cycles for each read in the sequencing run.
u Read 1—Enter a value up to 151 cycles.
u Index 1—Enter the number of cycles required for the Index 1 (i7) primer.
u Index 2—Enter the number of cycles required for the Index 2 (i5) primer.
u Read 2—Enter a value up to 151 cycles. This value is typically the same number of cycles as
Read 1.
The control software confirms your entries using the following criteria:
u Total cycles do not exceed the maximum cycles allowed
u Cycles for Read 1 are greater than the 5 cycles used for template generation
u Index Read cycles do not exceed Read 1 and Read 2 cycles
5 [Optional] If you are using custom primers, select the checkbox for the primers used. For more
information, see NextSeq Custom Primers Guide (document # 15057456) .
u Read 1—Custom primer for Read 1.
u Index 1—Custom primer for Index 1.
u Index 2—Custom primer for Index 2.
u Read 2—Custom primer for Read 2.
6 [Optional] Select the Advanced Settings button to change run parameters.
u From the Recipe drop-down list, select a recipe. Only compatible recipes are listed.
u Output folder location—Change the output folder location for the current run. Select Browse to
navigate to a network location.
u Included file—Select files to include in the Output Folder that can be useful if further analysis is
required. For example, manifest files and sample lists.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
23
NextSeq 550Dx Research Mode Instrument Reference Guide

illumina NextSeq 550Dx Specifications

General

Brand	illumina
Model	NextSeq 550Dx
Category	Laboratory Equipment
Language	English