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LOADING THE INCUBATOR, 315/317 SERIES FLO-THRU CO2 INCUBATORS:
Load the chamber, spacing the items as far apart as possible (for
maximum air circulation).
AIR/GAS RATIO, 315/317 SERIES FLO-THRU CO2 INCUBATORS:
Set the flowmeters to obtain the required air and CO2 flow—using the
lowest possible CO2 flow rate will help to conserve gas.
Use one of the following formulas to find the desired air-to-gas ratio:
R = % CO2 in the chamber atmosphere
A = Air flow in liters/minute
C = CO2 flow in liters/minute
Equation 1 (to be used if air and CO2 flow rates are known):
R = C x 100
A + C
Equation 2 (to be used if air flow rate and % CO2 are known):
C = RA liters/min.
100 - R
Equation 3 (to be used if CO2 flow rate and % CO2 are known):
A = 100C - RC liters/min.
R
Example 1: If a CO2 percentage of 15% is desired at a flow rate of 0.4
liters/min. of CO2, use Equation 3:
A = 100 (0.4) - 15 (0.4)
= 40 - 6
15 15
= 2.26 liters/min. of air
Example 2: If flow rates are set 0.1 liters/min. of CO2 and 3 liters/min. of
air, use Equation 1 to find percentage of CO2:
B = 0.1 x 100 = 3.2% CO2 in chamber
3.0 + 0.1 atmosphere
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