Macherey-Nagel NucleoBond Xtra Midi

Questions and Answers

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Christine HansonAug 17, 2025
What to do if NucleoBond[®] Xtra column filter clogs during filtration with Macherey-Nagel NucleoBond Xtra Midi?
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  John QuinnAug 17, 2025
  If the NucleoBond[®] Xtra column filter clogs during filtration when using Macherey-Nagel Laboratory Equipment, ensure the precipitate was resuspended before loading by inverting the crude lysate at least 3 times directly before loading. Also, ensure a complete precipitation step by mixing well after neutralization to completely precipitate SDS and chromosomal DNA. Do NOT attempt to purify lysate prepared from a culture volume larger than recommended for any given column size with standard lysis buffer volumes. Ensure the lysate was cleared completely by using a NucleoBond[®] Xtra column filter or centrifuge at higher speed or for a longer period. Also, make sure lysis treatment was not too harsh, and lyse in Buffer LYS for no more than 5 min.
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dedwardsAug 25, 2025
How to avoid genomic DNA contamination of plasmid DNA with Macherey-Nagel NucleoBond Xtra Midi Laboratory Equipment?
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  Jesse CraigAug 26, 2025
  If you observe genomic DNA contamination of plasmid DNA with Macherey-Nagel Laboratory Equipment, it may be because the lysate was mixed too vigorously or vortexed after lysis. To prevent this, invert the tube only 5 times and do not vortex after the addition of LYS.
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Luis AcostaAug 28, 2025
Why was alkaline lysis inefficient with Macherey-Nagel Laboratory Equipment?
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  Richard LozanoAug 29, 2025
  If the alkaline lysis was inefficient when using Macherey-Nagel Laboratory Equipment, it might be because too much cell mass was used. Refer to section 4.4-4.6 regarding recommended culture volumes and lysis buffer volumes. Check plasmid content in the cleared lysate (see Figure 6). Also, check Buffer LYS for SDS precipitation before use, especially after storage below 20°C. If necessary, incubate the bottle for several minutes at 30-40°C and mix well until SDS is redissolved.
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audrey12Sep 1, 2025
How to fix plasmid that did not propagate with Macherey-Nagel NucleoBond Xtra Midi?
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  Mrs. Emily NelsonSep 2, 2025
  If your plasmid did not propagate when using Macherey-Nagel Laboratory Equipment, check plasmid content in the cleared lysate (see Figure 6). Use colonies from fresh plates for inoculation and add selective antibiotic to plates and media. Also, estimate plasmid content prior to large purifications by a quick NucleoSpin[®] Plasmid or NucleoSpin[®] Plasmid QuickPure preparation.
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Gregory NealSep 5, 2025
Why is nucleic acid pellet opaque with Macherey-Nagel Laboratory Equipment?
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  Jose RodriguezSep 5, 2025
  If the nucleic acid pellet is opaque or white instead of clear and glassy when using Macherey-Nagel Laboratory Equipment, it is likely due to co-precipitation of salt or residual alcohol. To resolve this, wash the pellet again with 70% ethanol, or increase the reconstitution buffer volume.
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cindyrothSep 9, 2025
What to do if DNA is degraded with Macherey-Nagel NucleoBond Xtra Midi?
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  Renee AllisonSep 9, 2025
  If your DNA is degraded when using Macherey-Nagel Laboratory Equipment, ensure that your entire equipment (pipettes, centrifuge tubes, etc.) is clean and nuclease-free. Also, do not lyse the sample with Buffer LYS for more than 5 min.
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Kayla HernandezNov 12, 2025
Why is there no or low plasmid DNA yield with Macherey-Nagel NucleoBond Xtra Midi Laboratory Equipment?
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  Kayla SmithNov 12, 2025
  If you are experiencing no or low plasmid DNA yield with your Macherey-Nagel Laboratory Equipment, it could be due to SDS or other precipitates in the sample. To resolve this, load the crude lysate onto the NucleoBond[®] Xtra column filter inserted in the NucleoBond[®] Xtra column to ensure complete removal of SDS precipitates. If precipitate is visible, it is recommended to filter or centrifuge the lysate again directly before loading it onto the NucleoBond[®] Xtra column. Another cause could be that the sample/lysate is too viscous. In this case, too much cell mass was used, so refer to section 4.4-4.6 regarding recommended culture volumes and lysis buffer volumes. Also, the pH or salt concentrations of buffers may be too high. To solve this, check plasmid content in the wash fractions (...
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lkhanNov 20, 2025
How to prevent RNA contamination of plasmid DNA with Macherey-Nagel NucleoBond Xtra Midi Laboratory Equipment?
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  Sean RollinsNov 20, 2025
  If you experience RNA contamination of plasmid DNA when using Macherey-Nagel Laboratory Equipment, it may be due to inefficient RNase digestion. Add new RNase to Buffer RES, as the RNase may not have been added to Buffer RES or was stored improperly. Also, the pH or salt concentration of the wash buffer may be too low. Check RNA content in the wash fractions (see Figure 6). Keep all buffers tightly closed. Check pH of Buffer EQU (pH 6.5) and WASH (pH 6.5) and adjust with HCl or NaOH if necessary. Ensure the NucleoBond[®] Xtra column filter was removed before the second washing step with Buffer WASH. Also, confirm that Buffer EQU was used instead of Buffer WASH for the first wash, as Buffer EQU has to be used to wash out the NucleoBond[®] Xtra column filter to avoid SDS carryover. If only m...

Macherey-Nagel NucleoBond Xtra Midi Specifications

General

Product Type	Plasmid DNA Purification Kit
Technology	Anion-exchange chromatography
Material	Silica
Application	Plasmid DNA isolation from E. coli cultures
Sample Input	Up to 250 ml of bacterial culture
Yield	Up to 500 µg
Recommended Plasmid Size	Up to 25 kb

Macherey-Nagel NucleoBond Xtra Midi - User Manual

Table of Contents

Questions and Answers

Macherey-Nagel NucleoBond Xtra Midi Specifications