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General| Format | Spin column |
|---|---|
| Article No. | 740727.10 |
| Number of preps | 10 |
| Catalog Number | 740727.10 |
| Sample Type | Genomic DNA |
| Storage Conditions | Room temperature |
| Sample Material | Cells, Tissue |
| Typical fragment size | 50 bp to 50 kb |
| Elution Volume | 50 - 100 µl |
Lists items required from the user for the procedure.
Guidance on how to effectively use the user manual.
Explains the scientific mechanism behind the purification method.
Details the technical parameters and capabilities of the kit.
Guidelines for cultivating bacterial cells for optimal results.
Different methods for eluting the purified DNA.
Lists hazard symbols, risk phrases (R-Sätze), and safety phrases (S-Sätze).
Details hazardous components with GHS symbols, H-phrases, and P-phrases.
Step 1: Preparing bacterial cells for lysis.
Step 2: Releasing plasmid DNA from bacterial cells.
Step 3: Removing cellular debris from the lysate.
Step 4: Loading DNA onto the purification column.
Step 5: Washing the column to remove contaminants.
Step 6: Releasing the purified plasmid DNA from the column.
Solutions for common problems and issues encountered.
Product codes, pack sizes, and catalog numbers for reordering.
Citations for scientific literature supporting the method.
Legal terms, usage limitations, and warranty information.
