Holding crossbar, position
Petrifilm Yeast & Mould spreader over
Petrifilm count plate.
10
Apply pressure on spreader to
distribute inoculum over circular
area. Do not twist or slide the
spreader.
11
Lift spreader. Wait one minute for gel
to solidify.
12
Incubate Petrifilm count plates with
the clear side up in stacks of 20 or
less at a temperature of 25°C
for 3-5 days.
Incubation Interpretation
13
Read Petrifilm count plates on a
standard Quebec-type colony
counter or other magnified light
source. Refer to Guide to
Interpretation when reading
results.
14
• Note: Remember to inoculate and spread each Petrifilm plate before going on to the next.
• Steps 9 and 10 are unique to Petrifilm Yeast & Mould count plates.
• Incubate Petrifilm Yeast & Mould count plates in a plastic container or plastic bag to optimize colony development.
Additional Comments
Europe
Laboratoires 3M Santé
Boulevard de l'Oise
95029 Cergy Pontoise Cedex
France
Tel.: +33 (0)1 30 31 85 71
Fax: +33 (0)1 30 31 85 78
Dealer's stamp
3
Microbiology Products
3M Health Care Ltd
3M House
Morley Street
Loughborough
Leicestershire LE11 1EP
Tel: (01509) 613181
Fax No. (01509) 613087
www.3M.com/microbiology
3M and Petrifilm are trademarks of the 3M Company
Whirl-Pak is a trademark of Nasco
© 3M Health Care Limited 2006
Date Version
January 2006 1.0
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