Mezzo ESP-7501 - Procedural Guidelines; Storage and Sample Preparation; Inoculation and Incubation Steps

Reminders
for use
Petrifilm
3M
™
Petrifilm
™
Yeast & Mould Count Plates
For detailed warnings, cautions, disclaimer of warranties / limited remedy, limitation of 3M liability,
storage and disposal information and instructions for use, see product's package insert.
Refrigerate unopened packages.
Use before expiration date on
package.
Storage
1
Petrifilm
To seal opened package, fold end
over and tape shut.
2
Petrifilm
Keep resealed package at ≤21°C
(≤70°F), ≤50%RH. Do not
refrigerate opened packages. Use
Petrifilm count plates within one
month after opening.
3
Prepare a 1: 10 or greater dilution
of food product.* Weigh or pipette
food product into Whirl-Pak
®
bag, stomacher bag, dilution
bottle or other appropriate sterile
container.
Sample Preparation
4
Add appropriate quantity of diluent.
These include Standard Methods
phosphate buffer, 0.1% peptone
water, distilled water, phosphate
buffered saline, and Butterfield's
buffer. Do not use buffers
containing sodium citrate or
thiosulfate.
5
Blend or homogenize sample per
current procedure.
* If greater sensitivity is required
with dairy or juice products please
refer to Petrifilm Dairy & Juice
Products sheet.
6
Place Petrifilm count plate on flat
surface. Lift top film.
Inoculation
7
With pipette perpendicular to
Petrifilm count plate, place 1ml of
sample onto centre of bottom film.
8
Release top film; allow it to DROP.
DO NOT roll top film down.
9