Microscopic Differentiation
Yeasts and moulds are very
diverse organisms, and cannot
always be distinguished from each
other macroscopically. As with
any method, positive
differentiation can be made with
microscopic examination.
To isolate colonies for further
identification, lift the top film and pick
the colony from the gel.
branching, thread-like filaments
(mycelium) – MOULD
or MOULD in various stages of
germination.
Look for oval shaped, budding
YEAST
Transfer the colony to a drop of sterile
water on a microscope slide, cover with
a coverslip, and view under oil
immersion power.
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