Rotary Microtome HM 325
MICROM International GmbH
Robert-Bosch-Str. 49
D- 69190 Walldorf 387821 - English
4-2 HOW TO AVOID MALFUNCTIONS
Specimen preparation:
• When preparing specimens, be sure that a
suitable embedding medium, fixation,
dehydration and infiltration time are chosen.
Specimen temperature:
• Sectioning is carried out at ambient
temperature (excluding frozen sections).
• If the temperature is too high, the paraffin
softens. Therefore, avoid heating paraffin
specimens by direct exposure to sunlight or
other near sources of heat.
Tightening the clamping screws:
• Tighten all clamping screws and clamping
levers on the knife carrier, specimen holder
and specimen orientation.
Selection of the knife:
• Carefully select the required knife material
and profile.
Adjustment of the knife:
• Take care to adjust the proper clearance
angle of the knife: 5 - 7° for glass knives. For
diamond knives ask the knife manufacturer.
• Select a clearance angle adjustment of 5 - 15°
according to the facet angle. Typically, adjust
an angle of 10 - 12 °.
• Take care to adjust knife height.
Cutting speed:
Take care to select proper cutting speed.
• General Rule
: The harder the material, the
slower the cutting speed!
Trimming:
• Take care in bringing knife and specimen
together.
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