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Miltenyi Biotec UltraMicroscope Blaze - User Manual

Miltenyi Biotec UltraMicroscope Blaze
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UltraMicroscope Blaze Imaging System
User manual
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Summary

Important Safety Information

General Safety Instructions

Provides general safety instructions for personal safety and correct instrument use.

Important Safety Information

Hazard Levels

Explains signal words like WARNING, CAUTION, NOTICE for hazard communication.

Safety Symbols

Lists and defines various safety symbols used on the instrument and in the manual.

Important Safety Information

Safety Labels

Instructions on observing, cleaning, inspecting, and replacing safety labels.

Important Safety Information

Electrical and Thermal Hazards

Details hazards related to electricity, short circuits, overheating, fire, and explosion.

Protective Housing and Power Supplies

Covers safety of protective housing, cables, and power supplies.

Liquids Inside the Instrument

Warns about liquids causing short circuits and how to handle them.

Important Safety Information

Humid and Dusty Environments

Addresses hazards from humidity, condensation, or dust and precautions for use.

Important Safety Information

Biological Hazards

Covers contamination or infection risks from biological material and necessary precautions.

Chemical Hazards

Warns about hazardous substances and reagents and necessary safety measures.

Important Safety Information

Motorized Turret Safety

Safety precautions related to the movement of the motorized turret.

Optical Radiation and Laser Hazards

Warns about laser beams, potential eye injury, and safety practices.

Important Safety Information

Ergonomic Hazards

Addresses risks of falling instruments causing injury and heavy instrument handling.

Environmental Hazards

Covers tripping and slipping hazards in the work environment.

Servicing and Transportation Safety

Discusses hazards from improper servicing and safe transportation practices.

Important Safety Information

Instrument Transportation

Guidelines for careful transport of the instrument to prevent internal damage.

Equipment Disposal

Information on proper disposal of electrical and electronic equipment (WEEE).

Introduction

The UltraMicroscope Blaze Imaging System

Overview of the UltraMicroscope Blaze™ system, its capabilities, and features.

Intended Use

Defines the intended use, limitations, and user requirements for the instrument.

Introduction

Instrument Description

Describes the physical components and layout of the instrument.

Front and Rear Views

Details components visible from the front and rear of the instrument.

Introduction

Equipment Details

Describes specific equipment or controls, such as the jog wheel.

Installation

Components Included in Delivery

Lists and describes the components included in the delivery of the imaging system.

Installation

Optional Accessories

Lists optional accessories available for the UltraMicroscope Blaze™ Imaging System.

Installation

Consumables

Lists consumables that need to be ordered separately for the instrument.

Unpacking and Installation Procedures

Provides instructions and precautions for unpacking and installing the instrument.

Switching On and Off

Switching On the Instrument

Step-by-step guide for powering on the UltraMicroscope Blaze™ Imaging System and components.

Switching Off the Instrument

Step-by-step guide for powering off the UltraMicroscope Blaze™ Imaging System and components.

Graphical User Interface Overview

Overview of the icons and functions within the ImSpector software interface.

Preparing the Instrument

Biological Hazard Precautions

Emphasizes caution when handling samples and reagents due to biological hazards.

Instrument Power Check

Instruction to verify the instrument is powered on before proceeding.

Objective Lens Focus to Park Position

Guides the user to move the motorized turret to its resting position.

Cuvette Handling and Filling

Instructions for removing, filling, and re-seating the cuvette.

Preparing the Instrument

Cuvette Filling with Syringe

Details using a syringe to fill the cuvette to a specific level.

Cuvette Lift and Drawer Operation

Instructions for lifting the cuvette and closing the drawer.

Laser Alignment

Light Sheet Alignment

Explains how to align light sheets for optimal image contrast and resolution.

Setting the Beam Path

Provides instructions and safety warnings for setting up the laser beam path.

Laser Alignment

Alignment Tool Setup

Guides placement of the alignment tool and selection of wavelengths.

Laser Transmission Control and Live Preview

Setting laser transmission and starting live preview for alignment.

Laser Alignment

Beam Path Alignment Through Pinhole

Procedure to align beams through the pinhole foil for precise calibration.

Objective Lens and Focus Adjustment

Selecting objective lens and moving turret for alignment.

Fine-Tuning Laser Transmission and Crosshair Alignment

Fine-tuning laser transmission and using crosshair for accurate beam alignment.

Light Sheet Properties Adjustment

Adjusting Sheet NA and other properties for optimal light sheets.

Laser Alignment

Light Sheet Width Adjustment

Adjusting Sheet Width to maximum for optimal light sheet properties.

Enabling Central Beam for Alignment

Activates only the central beam for alignment adjustments.

Aligning Central Beam to Pinhole

Procedure for aligning the central beam to the mask's pinhole.

Laser Alignment

Adjusting Horizontal Focus

Detailed steps for adjusting the horizontal focus of the light sheet.

Offset Calibration for Focus

Adjusts offset calibration for horizontal focus.

Maximizing Beam Intensity and Saving Settings

Maximizes beam intensity and saves the adjusted horizontal focus settings.

Laser Alignment

Adjusting Lateral Beams (Right-Hand Side)

Guides the adjustment of lateral laser beams on the right side.

Disabling Central Beam for Lateral Adjustment

Deactivates the central beam to work on lateral beams.

Adjusting Z-Direction of Lateral Beams

Adjusts the z-direction of lateral laser beams for alignment.

Enabling All Three Beams for Final Check

Re-enables all three beams for final alignment check.

Intensity Profile Analysis and Adjustment Verification

Defines ROI for intensity profile analysis and verifies adjustment.

Adjusting Central Beam (Left-Hand Side)

Provides steps for aligning the central beam on the left side.

Laser Alignment

Selecting Left Light Sheet

Selects the left light sheet for alignment.

Enabling Central Beam for Left-Side Alignment

Activates the central beam for left-side alignment.

Adjusting Height with Screw z1

Uses adjustment screw z1 for height alignment of the central beam.

Adjusting Lateral Beams (Left-Hand Side)

Guides the adjustment of lateral laser beams on the left side.

Disabling Central Beam for Left Lateral Adjustment

Deactivates central beam for lateral beam adjustment on the left.

Adjusting Z-Direction of Left Lateral Beams

Adjusts the z-direction of lateral beams on the left side.

Laser Alignment

Enabling All Beams for Left-Side Check

Re-enables all beams for final left-side alignment check.

Intensity Profile Analysis for Left Side

Defines ROI for intensity profile analysis on the left side.

Verifying Left-Side Alignment Quality

Verifies left-side alignment quality using the Brain button.

Returning Alignment Tool to Reference Position

Returns the alignment tool to its reference position.

Setting Up an Experiment

Capturing a Single-Color Z-Stack

Detailed steps for capturing a single-color z-stack image.

Aligning the Sample for Imaging

Instructions for aligning the sample within the cuvette for imaging.

Setting Up an Experiment

Device Selection for Z-Stack

Selects the XYZ table device for z-stack acquisition.

Autosave Configuration

Configures autosave settings and storage location for image data.

Optical Setup and Channel Configuration

Sets up objective lens, wavelengths, and laser power for imaging.

Light Sheet Setup and Live Preview

Chooses light sheet configuration and initiates live preview.

Setting Up an Experiment

Optimizing Measurement Settings

Fine-tuning settings for optimal image quality and contrast.

Region of Interest (ROI) and Contrast Adjustment

Defines ROI and adjusts image contrast/brightness.

Horizontal Focus and Light Sheet Properties

Displays and adjusts horizontal focus, Sheet NA, and Sheet Width.

Setting Up an Experiment

Capturing a Single Image

Step-by-step instructions for capturing a single image.

Capturing a Multi-Color Z-Stack

Guides on capturing multi-color z-stack images.

Device Selection for Multi-Color

Configures devices for multi-color acquisition.

Setting Up an Experiment

Autosave and Channel Settings for Multi-Color

Activates autosave and configures channels for multi-color imaging.

Laser Power and Chromatic Correction

Sets laser power per filter and corrects chromatic aberrations.

Capturing Image (Multi-Color)

Refers to the general image capture procedure for multi-color stacks.

Setting Up an Experiment

Capturing a Mosaic Image

Instructions for capturing mosaic images by stitching multiple fields of view.

Mosaic Acquisition Interface

Selects mosaic mode and accesses the mosaic acquisition interface.

Defining Mosaic Area

Defines the mosaic area by adjusting boundary dots.

Setting Up an Experiment

Setting Mosaic Overlap

Sets the overlap percentage between adjacent mosaic tiles.

Starting Mosaic Image Measurement

Refers to the general image capture procedure for mosaic images.

Maintenance

Instrument Servicing

Recommendation for periodic servicing and contact information for support.

Cleaning the Instrument Exterior

Instructions and precautions for cleaning the instrument's exterior.

Cleaning the Cuvette

Steps for properly cleaning the cuvette without damage.

Technical Data and Specifications

General Specifications

Lists general and operating specifications for the imaging system.

Technical Data and Specifications

EU Declaration of Conformity

Declares that the product conforms to relevant EU directives and standards.

Technical Support

Legal Notes

Limited Warranty Terms

Outlines the terms and conditions of the limited warranty for the product.

Legal Notes

Trademarks

Lists trademarks registered by Miltenyi Biotec and other companies.

Miltenyi Biotec UltraMicroscope Blaze Specifications

General IconGeneral
BrandMiltenyi Biotec
ModelUltraMicroscope Blaze
CategoryAll in One Printer
LanguageEnglish