U-28
CHAPTER 3
•
General Technical Considerations
4. Determining Lossnay Core Resistance to Bacterial Cross-
Contamination and Molds
Test Report
(1) Object
To verify that there is no bacterial cross-contamination from the outlet air to the inlet air of the Lossnay Core.
(2) Client
MITSUBISHI ELECTRIC CO. NAKATSUGAWA WORKS.
(3) Test Period
April 26, 1999 - May 28, 1999
(4) Test Method
The test bacteria suspension is sprayed in the outlet duct at a pressure of 1.5 kg/cm
2
with a sprayer whose
dominant particle size is 0.3 - 0.5 µm. The air sampling tubes are installed at the center of Locations A, B, C, D
(see diagram below), in the Lossnay inlet/outlet ducts so that the openings are directly against the air ow, and
then connected to the impingers outside the ducts. The impingers are lled with 100 mL physiological salt solution.
The airborne bacteria in the duct air are sampled at the rate of 10L air/minute for three minutes.
(5) Test Bacteria
The bacteria used in this test are as followed;
Bacillus subtilis: IFO 3134
Pseudomonas diminuta: IFO 14213 (JIS K 3835: Method of testing bacteria trapping capability of precision
ltration lm elements and modules; applicable to precision ltration lm, etc. applied to air or liquid.)
(6) Test Result
The result of the test with Bacillus subtilis is shown in Table 1.
The result of the test with Pseudomonas diminuta is shown in Table 2.
Sprayer
Impinger
Impinger
Impinger
Fan
Fan
Safety cabinet
Impinger
LOSSNAY Core
HEPA Filter
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