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MJ Research PTC-200 DNA Engine - User Manual

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DNA Engine Opticon
®
System
06678 revC.A
For Continuous Fluorescence Detection
PTC-200 DNA Engine
®
Cycler
CFD-3200 Opticon
Detector
Operations Manual
Supports Software Version 1.08
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Do you have a question about the MJ Research PTC-200 DNA Engine and is the answer not in the manual?

Summary

Introduction

Important Safety Information

Provides critical safety warnings and guidelines for operating the DNA Engine Opticon system.

Meet the DNA Engine Opticon System

Introduces the DNA Engine Opticon system and highlights its key features and benefits.

Layout and Specifications

Specifications

Details the technical specifications of the DNA Engine Opticon system, including thermal range, accuracy, and power.

Computer Specifications

Lists the minimum computer specifications required for operating the Opticon system.

Installation and Operation

Setting Up the DNA Engine Opticon System

Guides on connecting and setting up the DNA Engine Opticon system for the first time.

Environmental Requirements

Specifies the necessary environmental conditions for safe and optimal system performance.

Loading Sample Vessels into the Block

Instructions on how to properly load sample vessels into the thermal cycler block.

Compatible Chemistries, Sample Vessels, and Sealing Options

Compatible Chemistries

Discusses compatible chemistries like SYBR Green I, molecular beacons, and probes for the Opticon system.

Selecting the Correct Sample Vessel

Provides guidance on choosing appropriate sample vessels and microplates for the system.

Introduction to Opticon Monitor Software

Launching and Navigating Opticon Monitor Software

Instructions on how to start and navigate the Opticon Monitor software interface.

How Opticon Monitor Software Works

Explains the three main phases of operation within the Opticon Monitor software.

Experimental Setup and Programming

Creating a Master File

Details the process of creating a master file to orchestrate experimental runs.

Creating a Protocol File

Guides on creating protocol files that define thermal cycling parameters and steps.

Temperature Control Method

Explains the two methods for controlling block temperature: Calculated and Block Control.

Run Initiation and Status

Running a Protocol

Instructions on how to initiate a run and save the acquired data.

Monitoring Run Status

Describes how to monitor the progress and status of an ongoing run.

Data Analysis

Quantitation

Explains how to calculate the quantity of template initially present in a sample.

Adjusting the Cycle Threshold Line

Details how to adjust the C(t) line for accurate quantitation analysis.

Melting Curve

Covers performing and analyzing melting curve data for product identification.

Maintenance

Cleaning the Chassis and Block

Provides instructions for cleaning the exterior and the sample block of the unit.

Changing the Fuses

Step-by-step guide on how to replace the unit's fuses.

Troubleshooting

Software Error Messages

Lists common software error messages, their causes, and suggested resolutions.

Recalibrating

Instructions on how to recalibrate the instrument if calibration is inaccurate.

MJ Research PTC-200 DNA Engine Specifications

General IconGeneral
BrandMJ Research
ModelPTC-200 DNA Engine
CategorySecurity Sensors
LanguageEnglish