NanoEnTek EVE PLUS - User Manual

NanoEnTek EVE PLUS

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User Manual

Automated cell counter

Copyright NanoEnTek, Inc. All rights reserved.

Questions and Answers

D
Dr. Randall WalshAug 3, 2025
Why is the NanoEnTek Laboratory Equipment cell count inaccurate?
- L
  Lori PowellAug 3, 2025
  An inaccurate cell count with your NanoEnTek Laboratory Equipment can occur for several reasons. Ensure you're not inserting the EVE™ Cell counting slide upside-down, as this can introduce damaging liquid. Avoid reusing EVE™ Cell counting slides, as leftover dye can affect readings. Do not use counting slides other than EVE™ Cell counting slides. Also, make sure the sample covers the entire counting area and the EVE™ Cell counting slide is fully inserted. If the sample is outside the recommended range, dilute or add more cells. When viewing cells in zoom mode, adjust the focus knob to ensure live cells have bright centers and dead cells have dark/blue centers. Ensure the cells are not clumped. It is also recommended to calibrate the counter each year to maintain instrument sensitivity.
A
Austin KingAug 12, 2025
What to do if NanoEnTek Laboratory Equipment has saving and printing problems?
- A
  Autumn ThomasAug 12, 2025
  If you're experiencing saving and printing issues with your NanoEnTek Laboratory Equipment, use the USB drive supplied with the counter or a USB 2.0 drive, as other types may not be detected. Avoid saving too many files on the USB drive, as this can slow down the counter. Do not remove the USB drive or turn off the counter during updates. Also, do not remove the USB drive when saving or reading data, as it may damage the counter.
G
Gregory RhodesAug 23, 2025
How to fix NanoEnTek EVE PLUS when instrument not updating software?
- C
  csanchezAug 23, 2025
  If your NanoEnTek Laboratory Equipment is not updating software, the issue may stem from a corrupted software file or a damaged USB drive. Try downloading a fresh version of the software onto a different USB drive and then attempt the update again.

Summary

Install EVE PLUS

Activation Code

Setting Menu

Mode

Select count mode for cell counting (SINGLE CELL or CLUMP CELL).

Parameter

Parameter Details

Sensitivity

Minimum Cell Size

Maximum Cell Size

Circularity

Calibration

Recalibration Procedure

Mix Trypan blue 1:1 with buffer, load sample, insert slide, and press Start.

Recommended Actions

Cell Counting (Auto Focus)

Cell Counting (Manual Focus)

Data Export (Report & Image)

Save to USB via Main Screen

Save to USB via Data Tab

Data Export (CSV File)

Maintenance and Cleaning

Troubleshooting Inaccurate Cell Count

Sample Handling Issues

Do not insert the EVE™ Cell counting slide upside-down as this may introduce liquid into the instrument.

Low and High Readings

The EVE™ PLUS is designed to read samples from 1 x 104 cells/mL to 2 x 107 cells/mL.

Poor Image Quality

While viewing cells in the zoom mode, use the focus knob to adjust the image.

Clumpy Cell Issues

Ensure the cells are not clumped. Calibrate the counter each year.

Warranty Information

Product Specifications

EVE PLUS Instrument Specs

EVE Cell Counting Slide Specs

Safety Precautions

General Safety Instructions

Review and follow the safety instructions below: Do not install the instrument in a humid place.

Electrical Safety

To avoid potential shock hazard, make sure that the power cord is properly grounded.

Instrument Handling Precautions

Do not install an instrument on a slant or a place prone to vibrations.

Summary

Install EVE PLUS

Activation Code

Setting Menu

Mode

Select count mode for cell counting (SINGLE CELL or CLUMP CELL).

Parameter

Parameter Details

Sensitivity

Minimum Cell Size

Maximum Cell Size

Circularity

Calibration

Recalibration Procedure

Mix Trypan blue 1:1 with buffer, load sample, insert slide, and press Start.

Recommended Actions

Cell Counting (Auto Focus)

Cell Counting (Manual Focus)

Data Export (Report & Image)

Save to USB via Main Screen

Save to USB via Data Tab

Data Export (CSV File)

Maintenance and Cleaning

Troubleshooting Inaccurate Cell Count

Sample Handling Issues

Do not insert the EVE™ Cell counting slide upside-down as this may introduce liquid into the instrument.

Low and High Readings

The EVE™ PLUS is designed to read samples from 1 x 104 cells/mL to 2 x 107 cells/mL.

Poor Image Quality

While viewing cells in the zoom mode, use the focus knob to adjust the image.

Clumpy Cell Issues

Ensure the cells are not clumped. Calibrate the counter each year.

Warranty Information

Product Specifications

EVE PLUS Instrument Specs

EVE Cell Counting Slide Specs

Safety Precautions

General Safety Instructions

Review and follow the safety instructions below: Do not install the instrument in a humid place.

Electrical Safety

To avoid potential shock hazard, make sure that the power cord is properly grounded.

Instrument Handling Precautions

Do not install an instrument on a slant or a place prone to vibrations.

NanoEnTek EVE PLUS Specifications

General

Brand	NanoEnTek
Model	EVE PLUS
Category	Laboratory Equipment
Language	English