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Nikon Eclipse Ti Series User Manual

Nikon Eclipse Ti Series
19 pages
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Differential
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Contrast Attachment
Instructions
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Nikon Eclipse Ti Series Specifications

General IconGeneral
CategoryMicroscope
TypeInverted Microscope
IlluminationLED, Halogen, Metal halide
FocusMotorized
StageMotorized
AutomationYes
FocusingMotorized
NosepieceMotorized
SoftwareNikon NIS-Elements
CompatibilityVarious accessories and peripherals

Summary

Notes on Handling the System

About this DIC attachment

Explains the Senarmont System and the need for correct combinations of objective, condenser, and DIC prisms.

Combination for ∞L / ∞/∞/∞SS type

Details combinations for ∞L, ∞M, ∞H, ∞SS condenser cassettes based on objective NA and condenser lens.

Combination for N1 / N2 / NR type

Refers to Table A for combinations of objectives, DIC prisms, and condenser cassettes for N1, N2, NR types.

Chapter 1 Names of Parts

Chapter 2 Microscopy

Contrast Adjustment

Explains how to adjust contrast by rotating the polarizer and achieving sensitive gray background.

Shearing Direction and Contrast

Explains how contrast is obtained in the shearing direction and how to align the specimen.

DIC Microscopy with High-Magnification Objectives

Details focusing and re-adjusting the field aperture diaphragm for high-magnification observation.

Chapter 3 Principles of DIC Microscopy

Basic Structure and Phase Change

Describes the basic structure and how subjects cause phase changes in light.

Diagram of DIC Principles

Illustrates the light path through polarizers and Wollaston prisms in DIC microscopy.

Comparison of DIC Microscopy and Phase Contrast Microscopy

Compares DIC and Phase Contrast microscopy characteristics, sensitivity, and suitability for specimens.

Chapter 4 Assembly

Optical System Direction Adjustment (Adjustment of vibration direction)

Guides the adjustment of polarizer and analyzer vibration direction for optimal contrast.

DIC polarizer alignment

Details aligning the DIC polarizer's white index mark with the dial scale.

Condenser and polarizer alignment for dark cross

Guides aligning condenser and polarizer to achieve a dark cross in the objective's exit pupil.

Chapter 5 Troubleshooting Tables

Contrast not obtained when using the DIC method

Lists causes and countermeasures for lack of contrast in DIC method.

Poor viewing or contrast

Lists causes and countermeasures for poor viewing or contrast issues.

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