The Opentrons OT-2 is an open-source liquid handling lab robot designed for scientists, offering high customizability and compatibility with various kits and reagents. It occupies half a lab bench and features software and hardware modules that do not require coding knowledge to operate. The device has completed certifications including CE & FCC, NRTL, CB, and ISO 9001.
Function Description:
The OT-2 is primarily a liquid handling robot, capable of performing automated pipetting tasks in a laboratory setting. It supports both single and multi-channel pipetting configurations, with two pipette mounts allowing for either one or two single-channel pipettes, or one or two 8-channel pipettes. These pipettes are easily interchangeable, providing flexibility for different experimental needs.
Important Technical Specifications:
- Dimensions:
- Height: 66 cm (26 inches)
- Width: 63 cm (25 inches)
- Depth: 57 cm (22.5 inches)
- Weight: Over 40 kg (requires two people for lifting).
- Gantry: Features a 0.1mm resolution XYZ gantry for precise movement.
- Pipette Volumes:
- Single-Channel: 1-1000 µl
- 8-Channel: 1-300 µl
- Frame Composition: Constructed with rigid steel and CNC aluminum for durability and stability.
- Enclosure: Made of polycarbonate.
- Deck: Features 11 deck slots compatible with standard SBS dimensions and includes a removable trash bin. Adapters are available to fit existing hardware on the deck. Modules snap into place.
- Power Requirements:
- Input: 100-240 VAC / 50-60 Hz
- Max Power Consumption: 220 W
- Connectivity:
- Wi-Fi: 2.4 GHz IEEE 802.11b/g/n
- USB: 2.0
- Ethernet
- Operating Temperature & Relative Humidity:
- Recommended Temperature: 20-24°C
- Recommended Relative Humidity: 40-60% RH
- Plate Type Compatibility:
- Supports 96- or 384-well plates.
- 96-well plates are recommended for optimal deck compatibility and touch tips.
- Compatible with up to 384-well plates.
- Software Requirements (for Opentrons App):
- Windows 10
- macOS 10.10 or later
- Ubuntu 12.04 or later
Usage Features:
- Ease of Setup: The Quickstart Guide provides step-by-step instructions for unboxing and initial setup, including removing packaging, installing window covers, and connecting power and data cables.
- User Interface: The Opentrons App (downloadable from opentrons.com/ot-app/) guides users through connecting to the OT-2, attaching pipettes, and calibrating the robot.
- Labware Compatibility: Users can utilize common labware from the Opentrons Labware Library. For labware not in the library, users can add it using the Labware Creator or request assistance from the Opentrons team.
- Protocol Library: A shared Protocol Library (protocols.opentrons.com/) is available for users to access and share protocols.
- API Compatibility: The OT-2 interfaces with any product that has an API. Its open-source software allows users to make adjustments for integration with other products. Custom code work may be required for specific integrations, and users may need to write custom libraries and install software packages not shipped with the robot.
- Included Accessories: The OT-2 comes with a comprehensive toolkit including a hand screwdriver, wrench, Torx key, hex key set, Super Lube, various nuts and screws for assembly, a trash bin, and a calibration block. It also includes window covers, a power supply, regional IEC power cable, Ethernet cable, and an Ethernet-to-USB dongle for laptop connectivity.
Maintenance Features:
- Cleaning: The OT-2 can be cleaned and disinfected with alcohol, bleach, or standard household cleaners using a rag or paper towel. Users are advised to avoid contact with electronics. It's important to note that the OT-2 is not a sterile environment, and users may add their own UV lights or the Opentrons HEPA Module for sterilization.
- Safety during Cleaning: Before cleaning or maintenance, the machine must be turned off, and the power disconnected. Users should also pay attention to any warning labels.
- Preventative Maintenance: Routine preventative maintenance is recommended to ensure optimal functioning and prolong the robot's lifespan. Opentrons offers service plans, and users can contact Support for more information.
- Troubleshooting: In case of issues, users are encouraged to contact Opentrons Support (support@opentrons.com). The serial number, located on the bottom right side of the machine, should be provided when contacting support.
- Warranty: The OT-2 comes with a one-year warranty against manufacturer defects and is stress-tested to last a minimum of three years of full-time use.
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