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PerkinElmer Spotlight 200 User Manual

PerkinElmer Spotlight 200
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Spotlight 200
User’s Guide
MOLECULAR SPECTROSCOPY

Table of Contents

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PerkinElmer Spotlight 200 Specifications

General IconGeneral
BrandPerkinElmer
ModelSpotlight 200
CategoryMicroscope
LanguageEnglish

Summary

Warnings and Safety Information

Electrical Safety

Covers essential safety precautions regarding connecting the instrument to power, earthing, and fuse handling.

Mechanical Safety

Addresses safety concerns related to the motorized stage, warning against placing fingers near moving parts.

Lifting the Spotlight 200

Provides instructions and warnings for safely handling and moving the heavy microscope and spectrometer components.

Getting Ready to Use the Spotlight 200

Cooling the MCT Detector

Provides detailed step-by-step instructions for cooling the MCT detector using liquid nitrogen.

Setting up the Spotlight 200

Guides through the process of powering on the spectrometer, microscope, and stage controller, and launching the software.

Focusing the Microscope

Explains how to adjust the microscope's focus using the joystick or auto-focus for optimal sample viewing.

Focusing the image

Details the procedure for focusing the sample image, including using the Z-control and auto-focus options.

Techniques for Collecting Spectra

Collecting the Spectrum of a Thick Sample

Details how to acquire spectra from thick samples, including removing the lower cassegrain assembly for reflectance mode.

Collecting Spectra With the ATR Objective

Describes the Attenuated Total Reflectance (ATR) technique and its application for opaque or strongly absorbing samples.

Fitting the ATR Crystal Holder to the Microscope

Provides step-by-step instructions for installing the ATR crystal holder onto the microscope.

Centering the Crystal

Details the procedure for aligning the ATR crystal holder to ensure proper sample contact and spectral acquisition.

Checking the Infrared Alignment

Explains how to verify the correct alignment of the infrared beam with the ATR crystal for optimal energy levels.

Collecting Spectra

Manual ATR Objective

Covers collecting background and sample spectra using the manual ATR objective, including scanning lines and maps.

Analyzing Samples Using the ATR Imaging Accessory

Reflectance FT-IR Microspectroscopy

Maintenance

Replacing the Microscope Fuse

Provides step-by-step instructions for safely switching off and replacing the microscope's primary fuse.

Replacing the Stage Controller Fuse

Details the procedure for safely replacing the primary fuse in the stage controller unit.

Connecting the Microscope to the Spectrometer

Connections to the Stage Controller