PromoKine PK-CA724-488HTS User Manual

Cell Proliferation Kit III
(EdU; HTS)
Instruction Manual
Cat.No. PK-CA724-488HTS
PK-CA724-555HTS
PK-CA724-594HTS*
PK-CA724-647HTS*

PromoKine PK-CA724-488HTS Specifications

General

Brand	PromoKine
Model	PK-CA724-488HTS
Category	Laboratory Equipment
Language	English

Summary

Introduction and product description

Materials provided with the Kit and Storage Conditions

Required Material and Equipment not included in this Kit

Workflow

Preparation of the Stock Solutions

Prepare 20X EdU Solution

Details on preparing the 20X EdU stock solution from Component A.

Prepare Buffer Additive Stock Solution

Details on preparing the buffer additive stock solution from Component E.

Labeling of Cells with EdU

Optimizing Incubation and Time

Guidance on adjusting EdU concentration and incubation time for optimal labeling.

Staining Cell-surface Antigens with Antibodies (optional)

Cell Fixation and Permeabilization

Fixation and Permeabilization Protocol

Step-by-step procedure for fixing and permeabilizing cells.

EdU Detection

Preparation of Click Assay Cocktail

Instructions for preparing the click assay cocktail components.

Performing the Click Reaction

Steps for incubating cells with the assay cocktail and subsequent washing.

Staining Intracellular or Surface Antigens (optional)

Antibody Staining Protocol

Procedure for staining cells with antibodies for antigen detection.

Imaging and analysis

Fluorescence Quantification and Imaging

Methods for scanning plates and capturing fluorescence images.

Example of the data derived from an experiment using the EdU-based HTS Kit:

Ordering Information