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Qiagen QIAsymphony SP - User Manual

Qiagen QIAsymphony SP
216 pages
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December 2017
QIAsymphony
®
SP/AS
Consolidated Operating
Guide
For use with software versions 4.0 and 4.1
Version 1
QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden
GERMANY
R2
1112127

Table of Contents

Questions and Answers

  • S
    Samantha StoneAug 16, 2025
    What to do if I get a bar code reading error on my Qiagen QIAsymphony SP Desktop?
    • M
      maria86Aug 16, 2025
      If you encounter a bar code reading error on your Qiagen Desktop, it might be due to a few reasons. The sample tube could be incorrectly positioned, the bar code itself might be dirty, or the print quality of the bar code could be poor. To resolve this, try the following: ensure the sample tube is correctly positioned, clean the bar codes, check the bar code printout, or reduce the speed at which the sample tubes are loaded.
  • F
    Felicia TranAug 22, 2025
    How to resolve disk space overrun on Qiagen QIAsymphony SP Desktop?
    • P
      Pam MeltonAug 22, 2025
      If the Qiagen Desktop system detects less than 15% free disk space, it results in a disk space overrun. You can delete result files older than 10 days (this is the default setting) from the instrument. Select 'Yes' to free up file space and delete these older files.
  • T
    Theresa WhiteAug 25, 2025
    What to do if Qiagen QIAsymphony SP Desktop detects the wrong adapter type?
    • S
      Sandra LarsonAug 26, 2025
      If the Qiagen Desktop detects a rack on a slot that doesn't match the rack defined for the run in the software, you can either remove the rack from the slot or adjust the rack definition in the software.
  • C
    Christopher GonzalezAug 28, 2025
    What to do if Qiagen QIAsymphony SP Desktop detects a missing eluate rack?
    • T
      Tracy ChambersAug 28, 2025
      If a rack has been removed from a slot but wasn't removed in the software, the Qiagen Desktop will detect a missing eluate rack. You can either replace the missing rack on the slot or remove the rack in the software.
  • R
    Regina CrawfordAug 31, 2025
    How to correct errors in work lists on Qiagen Desktop?
    • J
      Jay MillsAug 31, 2025
      If there are errors in the work lists, ensure that the work lists contain a unique elution rack ID per batch and an elution rack ID for all samples. You can either change the required elution rack ID in the work list or define the batch so that the samples to be processed use just one elution rack ID.
  • K
    Keith RamirezSep 3, 2025
    How to handle an unexpected eluate rack detected by Qiagen QIAsymphony SP?
    • J
      Jonathan JohnsonSep 3, 2025
      If the inventory scan detects an unexpected eluate rack on a slot that isn't registered in the system, open the 'Eluate' drawer, define the elution rack type by selecting the elution slot, and assign an elution rack type from the list. Alternatively, remove the elution rack from the 'Eluate' drawer. Close the 'Eluate' drawer and run another inventory scan.
  • K
    Kurt PetersSep 5, 2025
    What to do if Qiagen Desktop detects a clot?
    • F
      frankmullinsSep 6, 2025
      If the Qiagen Desktop detects a clot in a sample that prevents transfer by the QIAsymphony SP, remove the clot(s) and process the sample in a new batch.
  • K
    kellyevansSep 9, 2025
    How to resolve rack file format incompatibility on Qiagen Desktop?
    • I
      imitchellSep 9, 2025
      If the rack file has a format that is incompatible with the QIAsymphony SP/AS instruments, ensure that the rack file has the correct format. Use the CSV Conversion tool of the QIAsymphony Management Console to do this.
  • S
    Shannon CrawfordSep 10, 2025
    How to fix insufficient sample volume error on Qiagen Desktop?
    • S
      Shannon DanielSep 12, 2025
      If you're getting an insufficient sample volume error on the Qiagen Desktop, the liquid available might not be enough for the assays or the number of replicates. Verify that the sample volume corresponds to what is defined in the Sample Rack Layout screen.
  • D
    Dr. Jon SnyderSep 12, 2025
    Why is cooling for slot not supported on my Qiagen Desktop?
    • A
      Ashley BrownSep 12, 2025
      Cooling for a slot might not be supported on your Qiagen Desktop if the selected elution rack type requires an adapter that hasn't been configured in the list of available adapters and holders. Ensure that all available adapters are listed in the Adapters AS list and configure the correct number of available adapters in the Configuration menu.

Summary

Safety Information and Precautions

Proper Use and General Safety

Outlines proper operation and general safety guidelines for the instrument.

Electrical Safety and Hazards

Details electrical safety requirements and potential hazards associated with the instrument.

Biological Safety and Chemical Handling

Addresses biological safety, handling of infectious agents, and chemical safety.

Mechanical, Heat, and Maintenance Safety

Highlights safety precautions related to mechanical hazards, heat, and maintenance.

Radiation Safety

Details safety measures related to UV and laser radiation hazards.

Startup Procedure and General Features

Powering On and Off

Covers procedures for switching the QIAsymphony SP/AS on, logging in, and logging out.

User Settings and Configuration

User Account Management

Explains user roles (Supervisor, Operator) and managing user accounts.

Handling Files and Data Transfer

QIAsymphony SP Instrument Features

Key Instrument Components

Details instrument features like the magnetic head, lysis station, and robotic arm.

Loading QIAsymphony SP Drawers and Worktable

Loading the Waste Drawer

Details the procedure for loading the waste drawer with its components.

Loading the Eluate Drawer

Explains the features and loading procedure for the eluate drawer.

Loading Reagents and Consumables Drawer

Covers loading consumables, reagent cartridges, buffer bottles, and accessory troughs.

Performing Inventory Scans (SP)

Starting, Pausing, and Stopping Runs

Describes how to start, pause, resume, and stop runs.

QIAsymphony SP Run Definitions

Defining a Batch;Run (Queuing)

Guides on defining a batch/run, including sample loading and Assay Control Sets.

QIAsymphony AS Features and Operation

QIAsymphony AS Basic Functions

Preparing and Executing Runs

Covers preparing runs, defining assay favorites, and integrated run setup.

Assigning Assays and Sample Positions

Details assigning assays and sample positions, including manual and work list methods.

Troubleshooting and Error Handling

Error Messages and Warnings

Describes how error messages appear and are indicated in the interface.

Software Help Boxes and Technical Services

Explains how to use software help and contact QIAGEN Technical Services.

Error Codes and Their Solutions

Lists common error codes and provides suggested solutions.

Maintenance Procedures

Qiagen QIAsymphony SP Specifications

General IconGeneral
Nucleic Acid TypesDNA, RNA
ThroughputUp to 96 samples
Sample TypesBlood, serum, plasma, tissue, cells, swabs
Elution Volume50 - 200 µL
Dimensions (W x D x H)Approximately 120 x 80 x 90 cm
Power Requirements100-240 V, 50/60 Hz
TypeAutomated sample preparation system
Automation LevelFully automated, walkaway operation