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BluePippin
Sage Science BluePippin - User Manual
92 pages
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DNA
Size S
election S
ystem
Operations
Manual
2
Table of Contents
Main Page
Default Chapter
3
Table of Contents
3
1 Introduction
6
System Overview
6
How the System Works
7
Collection Strategies
7
Collection Types
8
2 Keys to Successful Collections
9
Common Misconceptions
9
Improving Sample Yield
11
Improving Sample Yield for HMW DNA
11
3 Safety and Precautions
12
4 Unpacking and Installation
13
5 Workflow Summary
19
6 Sample Preparation
20
7 Programming Guide
21
Overview
21
Programming Summary
22
Selecting a Cassette Definition
24
Run Times
25
Assigning a Reference Marker - External Marker
26
Assigning Reference Markers - Internal Standards
27
Warnings and Indicators
28
8 Programming Size Selections - Tight, Range, and Timed Modes
30
Tight Mode - Programming Minimum Size Range Collections
30
Range Mode- Programming Broad Size Range Collections
31
Time Mode - Programming Timed Collections
33
9 Optical Calibration
34
10 Preparing a Cassette
38
Visually Inspect the Cassette
38
Prepare the Cassette for Loading
40
Continuity Test
41
11 Loading Samples
43
12 Running a Protocol
45
Overview
45
Starting a Run
46
Monitoring a Run
47
Log Files
49
13 Sample Collection
50
Overview
50
14 Sample Recovery and Yield
51
Overview
51
Intrinsic Sample Recovery on the Bluepippin
51
Improving Product Yield by Selecting a Wider Size Range
52
Improving Product Recovery with the Field Reversal
53
HMW DNA: Recommendations to Improve Yield
54
15 System Validation
55
16 System Options Tab
56
17 Running in Manual Mode
58
18 Analyzing Runs - Log Review Tab
60
19 Managing Files -- File Manager Tab
65
Overview
65
File Types
66
Transferring Files
67
20 Upgrading Software
69
Extracting the Files to a USB Flash Drive
69
Upgrading the Pippin Instrument Software
71
21 Preventative Maintenance - Rinsing Electrodes
73
Electrode Rinse Procedure
73
22 Warranty and Service
74
23 Ordering Information
75
24 Instrument Specifications
77
25 Gel Cassettes Specifications
78
3% Agarose, 90 Bp-250 Bp, Marker Q2 (Internal Standards)
79
2% Agarose, 100 Bp-600 Bp, Marker V1 (Internal Standards)
80
2% Agarose, 100 Bp-600 Bp, Marker M1 (External Marker)
81
Agarose, 250 Bp-1.5 Kb, Marker R2 (Internal Standards)
82
Agarose, High-Pass, Marker R2 (Internal Standards)
83
Agarose, 2-6 Kb, Marker S1, Low Voltage (External Marker)
84
Agarose, 1-6 Kb, Marker S1, Pulsed-Field (External Marker)
85
Agarose, 3-10 Kb, Marker S1, Pulsed-Field (External Marker)
86
Agarose, High-Pass Protocols (4-20Kb Thresholds)
87
Agarose, High-Pass Protocols (30-40Kb Thresholds)
88
Agarose, 10-18 Kb, Marker U1, Pulsed-Field (External Marker)
89
Agarose, 18-27 Kb, Marker T1, Pulsed-Field (External Marker)
90
Agarose, 50Kb Target, Marker Z1, Pulsed- Field (External Marker)
91
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Sage Science BluePippin Specifications
General
Brand
Sage Science
Model
BluePippin
Category
Laboratory Equipment
Language
English
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