Sakura Tissue-Tek Glas g2 Quick Reference Guide

Coverslipping
Tissue-Tek® Glas™ g2
Quick Reference Guide
Tissue-Tek® Glas™ g2
QRG | Glas g2 | 2010 | V-5 page 9 | 11
Table of coverslipped conditions and operator actions
Coverslipped Condition
Cause
Operator Action
Small air bubbles are
present.
Mounting medium is attached to the slide
transfer station. This causes a wrong angle
of the slide. Therefore the mounting
medium is not divided properly.
Remove the attached mounting medium by
dissolving it with xylene or an other solvent.
With certain mounting mediums air bubbles
enter if the coverslipping speed is low.
Decrease the coverslipping speed.
The tip of the dispensing nozzle was
soaked for an extended period of time in
the anti-drying bottle, so the viscosity of
the mounting medium in the tip of the
dispensing nozzle has dropped.
Press the PRIME key to dispense the
mounting medium.
The viscosity of the mounting medium in
the tip of the dispensing nozzle is high. The
amount of solvent in the anti-drying bottle
is low.
Press the PRIME key to dispense the
mounting medium. Adjust the amount of
solvent in the anti-drying bottle.
There is not enough or no clearing agent in
the loading station.
Add clearing agent to the loading station up
to the specified level.
The cover glass application pressure during
coverslipping is too weak.
Increase the coverglass application pressure.
For details, consult the CSC or Sakura dealer.
The starting position of the application of
mounting medium not correct.
Change the start position for application of
mounting medium. For details, consult the
CSC or the Sakura dealer.
The amount of mounting medium being
dispensed is not appropriate.
Change the amount of mounting medium for
the first dispense. For details, consult the
Customer Service Center or the Sakura
dealer.
Not enough clearing agent remains
attached to the slide (when the slide is
retrieved from the loading station).
Increase the slide retrieval speed. For details,
consult the CSC or Sakura dealer.
Mounting medium comes
out of the slide at the
frosted end, etc.
It is normal for mounting medium to come
out by several mm or so.
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The coverglass size does not match the
applicable settings on the system side.
Change the coverglass size displayed on the
screen.
Too much mounting medium is dispensed.
Decrease the amount of mounting medium
dispensed.
Too much clearing agent remains attached
to the slide when the slide is retrieved from
the loading station.
Decrease the slide retrieval speed. For
details, consult the CSC or Sakura dealer.
The cover glass application pressure during
coverslipping is too strong.
Decrease the coverglass application pressure.
For details, consult the CSC or Sakura dealer.
The start position for dispensing mounting
medium is not correct.
Change the start position for the dispensing
of mounting medium. For details, consult our
CSC or Sakura dealer.
The amount of mounting medium
dispensed during the first dispense is not
correct.
Change the amount of mounting medium for
the first dispense. For details, consult Sakura
dealer.
The back of the slide
becomes dirty with
mounting medium.
Mounting medium from the receiving rack
was transferred back to the back of the
slide.
Clean the receiving rack with solvent
(normally xylene).
The slide transfer stage is dirty with
mounting medium.
Clean the transfer stage with solvent
(normally xylene).
Too much clearing agent remains attached
to the slide.
Decrease the slide retrieval speed. For
details, consult the CSC or Sakura dealer.

Sakura Tissue-Tek Glas g2 Specifications

General

Brand	Sakura
Model	Tissue-Tek Glas g2
Category	Laboratory Equipment
Language	English