Concentration-Factor-Check Feature
When using entered standard solutions, the l/ab factor
is used to convert absorbance to concentration,
according to the equation
C=f*A
To verify that operating conditions do not vary
between reagent batches or from day to day, use the
concentration-factor-check features as follows: After
the concentration mode has been set up with standard
solutions, press the MODE select control until the
FACTOR LED lights, and read and record the factor
given on the digital display. Every time new standard
solutions are used for the same test (such as for a new
reagent batch or when setting up the instrument),
press the MODE select control until the FACTOR
LED lights, and note the factor on the digital display.
Concentration Measurements using FACTOR mode
on page 2-4 describes how to use the factor mode. For
more information on concentration measurements and
other techniques, contact Spectronic Instruments'
Application Laboratory and request a list of available
SpecTech Notes.
A change in the factor indicates a change in the slope
of the standard curve due to variation in operating
conditions. It is recommended that a standard always
be used to set the concentration mode. The operator
may choose, however, to set the blank to OOOA, then
switch to the concentration mode.
Note: It is not actually necessary to know the l/ab
factor because this factor is introduced into the
instrument when the concentration adjust
control is used to set the digital display to read
the concentration of the standard. See page 2-4
for detailed instructions.
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