SPT Labtech Apricot S3 - Page 5

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6. Create Protocol .......................................................................................... 49
6.1 – Aspirate .............................................................................................................. 50
6.1.1 – Varieties of Menus ...................................................................................... 50
6.1.2 – Station.......................................................................................................... 51
6.1.3 – Variables ...................................................................................................... 51
6.1.4 – Pause ........................................................................................................... 52
6.1.5 – Pre-Airgap and Post-Airgap ....................................................................... 52
6.1.6 – Increased Steps ........................................................................................... 52
6.1.7 – Preview Position ......................................................................................... 53
6.2 – Dispense ............................................................................................................. 54
6.2.1 – Multi-Dispensing Quadrant ....................................................................... 54
6.2.2 – Dispense
Speed Over 60uL ........................................................................ 54
6.2.3 – Disp
ense Location in Well .......................................................................... 54
6.2.4 – Side Touch ................................................................................................... 54
6.2.5 – Bottom Touch ............................................................................................. 55
6.2.6 – Decremental Steps ..................................................................................... 55
6.3 – Empty ................................................................................................................. 56
6.4 – Mix ...................................................................................................................... 57
6.4.1 Volume Retained ........................................................................................... 57
6.4.2 Mix at Corners ............................................................................................... 57
6.5 – Transfer .............................................................................................................. 58
6.6 – Serial Dilution ................................................................................................. 60
6.6.1 – Start Column and End Column
.................................................................. 60
6.6.2 – Interval ......................................................................................................... 60
6.6.3 – Preview Position ......................................................................................... 60
6.7 – F
ill Plate .............................................................................................................. 62
6.7.1 – Maximum Aspirate Volume ....................................................................... 62
6.7.2 – Remain Volume ........................................................................................... 62
6.7.3 – Preview Position ......................................................................................... 63
6.8 – Pooling ................................................................................................................ 64
6.9 – Wash ................................................................................................................... 65
6.9.1 – Using Pump ................................................................................................. 66
6.9.2 – Pre-Fill Period .............................................................................................. 66
6.9.3 – Post Wash Period ........................................................................................ 66
6.10 – Pause
................................................................................................................ 67