© 2021 SPT Labtech Ltd
SPT Labtech pipettors are highly accurate and precise systems. However, to achieve
optimum results, the end user should practice some speci
c programming and
pipetting techniques.
In some versions of the Software, the end user is responsible for adding
plates/reservoirs to the software database. These user de
ed settings will be
utilized when writing programs. Inaccurate settings can result in complications.
Please use the following guidelines for de
ning plates.
The bottom of the tip should be level with the top plane of the plate and centered
over the well. An accurate Well Top setting (along with the Well Bottom setting) will
help ensure greater accuracy when selecting the operation depth of a liquid handling
function.
The tip bottom should be just above the bottom of the well (not touching the bottom
surface). To set this, the user can drive the tip down, using small increments, until it
touches the plate. Then move up in small increments until the plate can slide or lift
slightly under the tip. The tips should not touch the well bottom when 100% depth is
selected in the liquid handling functions.
Note: When performing Tissue Culture, o
r similar assay
s which require the well’s
lower portion to be undisturbed, the user can program a Well-Bottom height that will
be above the samples. This will help prevent accidents in programming.
The Tip Touch function is designed to help draw liquid o the tip. The user should set
the Tip-Touch position to make contact with the well (the Tip-Touch setting should
not move the plate).
Note: When setting the Tip Touch position, consider the shape of the well.
For example, a V shaped well de
nition should be based on the desired depth (in the
plate) of the Tip Touch function in a program.
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