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STILLA Prism6 H24000.3 - User Manual

STILLA Prism6 H24000.3
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STILLA
Prism6 & Crystal Reader
software
for the
naica® system
User Manual
Prism6 H24000.3/H24000.6
Crystal Reader software v3.0.7.3

Table of Contents

Questions and Answers

  • H
    harrismichaelAug 20, 2025
    Why are there any or few analyzable droplets with no air bubble in my STILLA Prism6 H24000.3?
    • V
      Victoria KingAug 20, 2025
      To resolve the issue of any or few analyzable droplets with no air bubble in your STILLA Laboratory Equipment, ensure that the crucial partitioning step is included as the first step in your PCR program. If the problem continues, extract the live and system logs, referring to the section “How to use the Geode Software User Interface” in the Geode User Manual, and then contact Technical Support.
  • H
    haydencurtisAug 22, 2025
    What to do if the Crystal Reader software stops responding with STILLA Prism6 H24000.3 Laboratory Equipment?
    • C
      christopher15Aug 22, 2025
      If the Crystal Reader software application stops responding, go to “Control Panel,” then to “Task Manager,” and stop the Crystal Reader process. After that, restart the application.
  • J
    James BatesAug 25, 2025
    What does error message 'Prism6 instrument configuration not applicable with detection channel selection' mean for STILLA Laboratory Equipment?
    • C
      Clayton BradleyAug 25, 2025
      If you encounter the error message 'Prism6 instrument configuration not applicable with detection channel selection' while using STILLA Laboratory Equipment, note that depending on the Prism6 instrument configuration, up to 6 detection channels are available. For the H24000.3, it is a 3-color Prism6 configuration with detection in Blue, Green, and Red channels. The H24000.6 is a 6-color Prism6 configuration with detection in Blue, teal, Green, Yellow, Red, and Infra-red channels. Contact Technical Support to enable detection channels, if applicable.
  • R
    Robert ParkerAug 26, 2025
    How to fix large droplet coalescence area in the chamber of STILLA Prism6 H24000.3?
    • D
      Darrell RamirezAug 26, 2025
      To address the presence of a large droplet coalescence area in the chamber of your STILLA Laboratory Equipment, clean the foil of the chip with an anti-static spray and a lint-free wipe before loading the sample.
  • N
    nancy20Aug 27, 2025
    What causes droplet overlapping in STILLA Prism6 H24000.3 and how to fix it?
    • E
      evansjoshuaAug 28, 2025
      To resolve the issue of droplets overlapping (typically the bottom right corner of the chamber) in your STILLA Laboratory Equipment, note that stacked droplets are considered artifacts and are excluded from the analysis. To relax the crystal, bring the chips back to the Geode. In the menu, select Template > Protocols and launch the program named “Sapphire Protocol Unpacking Droplets”. After the reading step, the neural network of Crystal Miner should recognize more droplets.
  • R
    robert84Aug 29, 2025
    How to troubleshoot a red light in the third position on STILLA Prism6 Laboratory Equipment?
    • J
      Justin GardnerAug 30, 2025
      To resolve a red light appearing in the third position on the front of the Prism6 instrument, reboot the Prism6 instrument by pressing the power button on the front for a few seconds until it shuts down, then press it again to restart. Also, restart the Crystal Reader software.
  • S
    Shawn BurgessSep 1, 2025
    How to solve error with Image Analysis Configuration File (.yaml) on STILLA Prism6 H24000.3?
    • M
      Michelle MooreSep 1, 2025
      If you receive the error message: 'The current Image Analysis Configuration File (.yaml) is not designed to be used on this scanner type' with your STILLA Laboratory Equipment, use the scanning templates provided by Stilla Technologies, accessible at https://www.stillatechnologies.com/technical-resources/naica-system-prism6/. Ensure consistency between the “.yaml” file and the experiment settings when editing “.yaml” files.
  • H
    Haley MillerSep 3, 2025
    What to do if Crystal Miner Plot configuration file (“.ncp”) uses different channels than the ones activated in STILLA Prism6 H24000.3?
    • K
      Kristen MaddoxSep 3, 2025
      If you encounter the error message: 'The selected Crystal Miner Plot configuration file (“.ncp”) uses different channels than the ones activated' with your STILLA Laboratory Equipment, use the scanning templates provided by Stilla Technologies, accessible at https://www.stillatechnologies.com/technical-resources/naica-system-prism6/. Ensure consistency between the “.ncp” file and the experiment settings.
  • B
    Beth SkinnerSep 4, 2025
    How to resolve Spillover Compensation Matrix file (“.ncm”) error in STILLA Prism6 H24000.3 Laboratory Equipment?
  • J
    Jeffrey KnightSep 6, 2025
    Why do the droplets in the chamber have low background fluorescence in STILLA Prism6 H24000.3 Laboratory Equipment?
    • C
      Cassie BlairSep 7, 2025
      To address droplets in the chamber having low background fluorescence with STILLA Laboratory Equipment, verify that the appropriate amount of reference fluorophore (e.g., fluorescein at 100 nM) was included in the reaction mix.

Summary

Introduction to the Naica System

Purpose of the Document

States the manual's aim regarding Prism6 and Crystal Reader software usage.

Overview of Crystal Digital PCR Workflow

Explains Stilla Technologies' next-generation technology for nucleic acid quantification.

Prism6 Instrument Specifications and Setup

Prism6 Declaration of Conformity and Specifications

Presents technical specifications and compliance information for the Prism6 instrument.

Prism6 Installation Instructions

Outlines the necessary infrastructure and procedures for installing the Prism6 instrument.

Operating the Prism6 Instrument

Software and System Prerequisites

Lists the operating system, hardware, and software requirements for running the Crystal Reader software.

Prism6 Instrument Initialization

Guides users through the process of powering on and initializing the Prism6 instrument.

Chip Handling and Positioning

Instructions for Chip Positioning

Details how to correctly load chips into the Prism6 instrument using tray holders.

Crystal Reader Software Workflow

Instructions for Chip Scanning

Explains how to set up experimental conditions for image acquisition using the Crystal Reader software.

Experimental Setup Steps

Setting Up the Scan Experiment

Outlines the five main steps for setting up a chip scan experiment in the Crystal Reader software.

Experiment Details Configuration

Naming the Experiment

Guides users on how to enter a descriptive name for the experiment in the software interface.

Editing Experiment Details

Configure Experiment Parameters

Guides on inputting fluorophore, target names, and selecting detection channels for an experiment.

Scanning Parameters Configuration

Check Scanning Parameters

Explains how to adjust exposure times and focus values for optimal image acquisition.

Chamber Details and Context

Select Chambers and Define Context

Guides users on selecting chambers and defining sample details for scanning.

Launching the Scan Process

Launch the Scan

Details the process of initiating the chip scanning sequence after setup is complete.

Image Quality Assessment

Check Chamber Image Quality

Explains how to visually inspect acquired images and assess their quality using flags.

Detailed Image Exploration

Explore Chamber Images in Detail

Guides users on navigating and analyzing individual chamber images using software tools.

Advanced Instrument Functionalities

Rescanning Chambers and Chips

Explains how to re-scan existing or new chambers to update experimental data.

User Profile Settings

Edit Settings for User Profiles

Details how to configure user preferences and system settings within the Crystal Reader software.

Focus Calibration Procedure

How to Perform Focus Calibration

Guides users on calibrating the instrument's focus for optimal image sharpness.

Maintenance and Technical Support

Cleaning and Decontamination

Details instrument cleaning, decontamination, and contact information for technical assistance.

Troubleshooting Error Messages

Display Screen Error Messages

Lists common error messages and their recommended solutions for Prism6 and Crystal Reader software.

STILLA Prism6 H24000.3 Specifications

General IconGeneral
BrandSTILLA
ModelPrism6 H24000.3
CategoryLaboratory Equipment
LanguageEnglish

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