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THARMAC Cellspin II - User Manual

THARMAC Cellspin II
51 pages
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Instruction manual
Cellspin® II / Cellspin® IIR
Cytocentrifuge
Man CS2/CS2R 01.2017 EN

Table of Contents

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Summary

Use According to Specification

Remaining Risks

Technical Specifications

Safety Guidelines

General Safety Precautions

Covers safe installation, operation, material handling, and environmental restrictions for the centrifuge.

Personnel and Regulatory Compliance

Ensures operation by trained staff and adherence to legal and professional safety regulations.

Handling Hazardous Substances and Bio-Safety

Procedures for centrifuging hazardous materials, including bio-safety systems and containment.

Equipment and Maintenance Safety

Guidelines for using approved parts, authorized repairs, and compliance with electrical safety standards.

Symbol Meanings

Delivery Checklist

Unpacking the Centrifuge

Initial Operation

Opening and Closing the Lid

Rotor Installation and Removal

Loading the Rotor

Control and Display Elements

Control Knob

A rotary knob used for setting individual parameters by turning clockwise or anticlockwise.

Control Panel Pushbuttons

Keys for selecting parameters, starting/stopping runs, and accessing special functions like emergency stop.

Entering Centrifugation Parameters

Programming

Program Input;Alteration

Instructions for entering new programs or modifying existing ones through the control panel.

Program Recall

Procedure for selecting and displaying stored centrifugation programs.

Centrifugation

Centrifugation with Pre-set Time

Details on starting and performing a centrifugation run with a defined duration.

Continuous Run

How to operate the centrifuge in continuous run mode without a set time limit.

Short-Term Centrifugation

Instructions for performing brief centrifugation runs by holding down the start button.

Emergency Stop

Acoustic Signal and Operation Hours

Cooling Functions

Standby-Cooling

How the centrifuge maintains a set temperature when the rotor is at rest and the lid is closed.

Pre-cooling the Rotor

Procedure for cooling the rotor to a specific temperature before a centrifugation run.

Relative Centrifugal Force (RCF)

Centrifugation of High-Density Materials

Rotor Identification

Emergency Release

Maintenance and Servicing

Surface Cleaning and Care

Instructions for cleaning the centrifuge housing, lid, and centrifuging chamber with appropriate agents.

Surface Disinfection

Procedures for disinfecting the centrifugal chamber when contaminated with infectious materials.

Removal of Radioactive Contaminants

Steps for safely removing radioactive contaminants from the centrifuge and accessories.

Rotors and Attachments Care

Guidelines for cleaning, inspecting, and maintaining rotors and accessories for optimal performance and safety.

Disinfection of Rotors and Accessories

Methods for disinfecting rotors and accessories, including precautions for specific materials.

Autoclaving Procedures

Instructions for autoclaving compatible centrifuge accessories at specific temperatures and durations.

Centrifuge Container Handling

Procedures for dealing with broken centrifuge containers and glass splitters.

Troubleshooting Faults

Centrifuge Acceptance for Repair

Disposal Guidelines

Introduction to Cellspin Cytocentrifuge

Cellfunnel Preparation System Assembly

ECOfunnel Preparation System Assembly

Cleaning Reusable Consumables

Disinfection of Reusable Components

Procedures for disinfecting reusable Cellfunnel®, ECOfunnel®, and CellClips after use.

CellClip-Rotor and Seal Cleaning

Instructions for cleaning and disinfecting the closed CellClip-rotor and its seal.

Filter Card Disposal

Guidelines for the safe disposal of single-use filter cards due to potential contamination.

Removable Rotor 370 Operation

Closed CellClip-Rotor 101 Operation

Cleaning the Closed CellClip-Rotor

Application Fields

Body Fluids Processing

Guidelines for processing various body fluids, including urine, cysts, and cerebrospinal fluid.

Cytological Sample Processing

Methods for processing cytological samples from gynecological, urological, and internal medicine examinations.

Cell Culture and Pathology Samples

Procedures for processing cell cultures and pathology samples for cytocentrifugation.

Processing Body Fluids

Additional Information

Sample Liquid Quantity

Guidance on optimal sample liquid volumes for different preparation systems.

Cell Concentration Guidance

Recommendations on cell concentration and dilution for achieving desired results.

Centrifugation Parameters

Information on recommended centrifugation speeds, times, and acceleration for various sample types.

Preventing Inaccurate Results

No Cells Received

Troubleshooting steps when no cells are obtained after centrifugation, addressing blocked filter cards or preparation issues.

Abnormal Cells Not Detected

Guidance on detecting abnormal cells, which may settle at the bottom and require resuspension.

Insufficient Cells Received

Solutions for obtaining too few cells, including issues with dilution, sample volume, or slide placement.

Excessive Cells Received

Advice for managing situations where too many cells are obtained, including dilution recommendations.

Cell Flow and Adhesion Issues

Troubleshooting cell movement and adhesion problems, addressing fixation and suspension medium evaporation.

Cell Band Formation Issues

Addressing issues with cell band formation, including cell thickness, border dryness, and suspension medium.

Recommended Times and Speed

Declaration of Conformity

Speed and Force Conversion Table

Consumables

Single Cellfunnel Consumables

List of articles including reusable and disposable Cellfunnels®, filter cards, and Cytoslides for single preparation systems.

Double Cellfunnel Consumables

List of articles including reusable and disposable Double Cellfunnels®, filter cards, and Cytoslides.

ECOfunnel Consumables

Details on reusable and disposable ECOfunnels®, ECO-slides, and ECO-seals compatible with the system.

EASY Series Consumables

List of EASY series consumables, including filter cards and disposable sample chambers.

THARMAC Cellspin II Specifications

General IconGeneral
BrandTHARMAC
ModelCellspin II
CategoryLaboratory Equipment
LanguageEnglish