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Thermo Scientific EVOS M5000 - User Manual

Thermo Scientific EVOS M5000
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For Research Use Only. Not for use in diagnostic procedures.
EVOS
M5000 Imaging System
For Fluorescence and Transmitted Light Applications
Catalog Number AMF5000
Publication Number MAN0017563
Revision A.0

Table of Contents

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Summary

About this guide

Safety alert words

Defines the meaning of CAUTION, WARNING, and DANGER to indicate potential hazards.

Installation

Operating Environment and Site Requirements

Specifies the necessary environmental conditions and space for installing the imaging system.

Remove Shipping Restraints

Explains how to safely remove shipping restraints before powering on the instrument.

Capture Images

Adjust Brightness

Explains how to optimize image brightness using Simple or Actual modes and their controls.

Focus on the Sample

Describes methods for achieving optimal focus using manual controls or autofocus.

Show Cell Count

Run Auto Count

Details the steps for performing an automated cell count using defined parameters.

Perform Manual Count

Explains how to manually tag and count cells using custom labels in multiple channels.

Capture and Save Time Lapse Images

Time Lapse Tool

Introduces the Time Lapse tool for capturing images at defined intervals over time.

Run a Time Lapse Routine

Guides on setting up and running a new time lapse experiment, including channels and intervals.

Capture Z-Stack

Z-Stack Tool

Introduces the Z-Stack tool for capturing multiple focal planes to increase depth of field.

Capture Z-Stack Images

Guides on defining Z-Stack parameters, including focus positions and step size.

Adjust Instrument Settings

Calibrate Objective Magnification

Explains how to calibrate objective magnification for accurate field of view and parfocality.

Calibrate White Balance

Guides on calibrating color channel lighting for accurate color reproduction.

Instrument Care and Maintenance

Decontamination Procedures

Outlines steps for decontaminating the instrument after exposure to hazardous materials.

Change EVOS LED Light Cubes

Guides on safely replacing LED light cubes to customize system functionality.

Change the Objectives

Explains the procedure for removing and installing different objectives.

Calibrate the Objectives

Details the process for calibrating objective magnification, parfocality, and parcentration.

Appendix A: Troubleshooting

Image Quality Issues

Lists common image quality problems and their potential solutions.

Software Interface Issues

Addresses problems related to software controls, response, and network connectivity.

Appendix B: Graphical User Interface (GUI)

Capture Tab

Provides a detailed breakdown of all controls and functions within the Capture tab.

Show Cell Count

Explains the Auto Count and Manual Count features for quantifying cells.

Z-Stack

Describes the Z-Stack tool for capturing multi-plane images and generating projections.

Appendix D: Safety

Safety Conventions Used in This Document

Defines symbols and terms used to convey safety information and potential hazards.

Safety Alert Words

Explains the meaning of CAUTION, WARNING, and DANGER to indicate hazard levels.

General Instrument Safety

Provides essential safety guidelines for operating and handling the instrument.

Chemical Safety

Chemical Hazard Warning

Highlights chemical hazards and advises consulting Safety Data Sheets (SDS) before use.

Chemical Waste Safety

Chemical Waste Hazard

Identifies chemical waste hazards and refers to SDS and local regulations for disposal.

Waste Disposal

Outlines procedures for characterizing and disposing of chemical waste safely and compliantly.

Electrical Safety

Physical Hazard Safety

Moving Parts

Warns about the danger of moving parts and advises keeping hands clear during operation.

Biological Hazard Safety

Addresses biohazard risks associated with biological samples and provides safety guidelines.

Safety and Electromagnetic Compatibility (EMC) Standards

Summary

About this guide

Safety alert words

Defines the meaning of CAUTION, WARNING, and DANGER to indicate potential hazards.

Installation

Operating Environment and Site Requirements

Specifies the necessary environmental conditions and space for installing the imaging system.

Remove Shipping Restraints

Explains how to safely remove shipping restraints before powering on the instrument.

Capture Images

Adjust Brightness

Explains how to optimize image brightness using Simple or Actual modes and their controls.

Focus on the Sample

Describes methods for achieving optimal focus using manual controls or autofocus.

Show Cell Count

Run Auto Count

Details the steps for performing an automated cell count using defined parameters.

Perform Manual Count

Explains how to manually tag and count cells using custom labels in multiple channels.

Capture and Save Time Lapse Images

Time Lapse Tool

Introduces the Time Lapse tool for capturing images at defined intervals over time.

Run a Time Lapse Routine

Guides on setting up and running a new time lapse experiment, including channels and intervals.

Capture Z-Stack

Z-Stack Tool

Introduces the Z-Stack tool for capturing multiple focal planes to increase depth of field.

Capture Z-Stack Images

Guides on defining Z-Stack parameters, including focus positions and step size.

Adjust Instrument Settings

Calibrate Objective Magnification

Explains how to calibrate objective magnification for accurate field of view and parfocality.

Calibrate White Balance

Guides on calibrating color channel lighting for accurate color reproduction.

Instrument Care and Maintenance

Decontamination Procedures

Outlines steps for decontaminating the instrument after exposure to hazardous materials.

Change EVOS LED Light Cubes

Guides on safely replacing LED light cubes to customize system functionality.

Change the Objectives

Explains the procedure for removing and installing different objectives.

Calibrate the Objectives

Details the process for calibrating objective magnification, parfocality, and parcentration.

Appendix A: Troubleshooting

Image Quality Issues

Lists common image quality problems and their potential solutions.

Software Interface Issues

Addresses problems related to software controls, response, and network connectivity.

Appendix B: Graphical User Interface (GUI)

Capture Tab

Provides a detailed breakdown of all controls and functions within the Capture tab.

Show Cell Count

Explains the Auto Count and Manual Count features for quantifying cells.

Z-Stack

Describes the Z-Stack tool for capturing multi-plane images and generating projections.

Appendix D: Safety

Safety Conventions Used in This Document

Defines symbols and terms used to convey safety information and potential hazards.

Safety Alert Words

Explains the meaning of CAUTION, WARNING, and DANGER to indicate hazard levels.

General Instrument Safety

Provides essential safety guidelines for operating and handling the instrument.

Chemical Safety

Chemical Hazard Warning

Highlights chemical hazards and advises consulting Safety Data Sheets (SDS) before use.

Chemical Waste Safety

Chemical Waste Hazard

Identifies chemical waste hazards and refers to SDS and local regulations for disposal.

Waste Disposal

Outlines procedures for characterizing and disposing of chemical waste safely and compliantly.

Electrical Safety

Physical Hazard Safety

Moving Parts

Warns about the danger of moving parts and advises keeping hands clear during operation.

Biological Hazard Safety

Addresses biohazard risks associated with biological samples and provides safety guidelines.

Safety and Electromagnetic Compatibility (EMC) Standards

Thermo Scientific EVOS M5000 Specifications

General IconGeneral
BrandThermo Scientific
ModelEVOS M5000
CategoryAll in One Printer
LanguageEnglish

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