[ CARE AND USE MANUAL ]
1
CONTENTS
I. GETTING STARTED
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
II. COLUMN USE
a. Sample Preparation
b. Operating pH Limits
c. Solvents
d. Pressure
e. Temperature
III. SCALING UP/DOWN ISOCRATIC METHODS
IV. TROUBLESHOOTING
V. COLUMN CLEANING, REGENERATING,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI. CONNECTING THE COLUMN
TO THE HPLC SYSTEM
a. Column Connectors and
System Tubing Considerations
b. Measuring System Band Spreading Volume
c. Measuring Gradient Delay Volume
(or Dwell Volume)
VII. eCORD INTELLIGENT CHIP TECHNOLOGY
(applies only to XBridge XP 2.5 m,
≤4.6 mm I.D. Columns )
Thank you for choosing a Waters® XBridge® Peptide BEH
C
18
, 130Å or 300Å Column. The XBridge Peptide BEH C
18
,
130Å and 300Å packing materials were designed to provide
excellent peak shape, high efficiency, and excellent stability.
The XBridge Peptide BEH C
18
, 130Å and 300Å packing materials
are manufactured in a cGMP, ISO 9002 certified plant using
ultra pure reagent. Each batch of XBridge Peptide BEH C
18
Column material has been qualified with a peptide separation,
and the results are held to narrow specification ranges to
assure excellent, reproducible performance for peptide
separations. Every column is tested and a Performance Test
Chromatogram, along with a Certification of Acceptance, are
provided with each column.
XBridge Peptide BEH C
18
, 130Å and 300Å Columns
VIII. ADDITIONAL INFORMATION
a. Use of Narrow-Bore (3.0 mm I.D.)
b. Impact of Band Spreading Volume
on 2.1 mm I.D. Column Performance
c. Non-Optimized vs. Optimized
LC-MS/MS System:
System Modification Recommendations
IX. REPRESENTATIVE TEST CHROMATOGRAPH
AND CONDITIONS FOR SEPARATION OF
PROTEIN DIGEST
X. CAUTIONARY NOTE
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