Zymo Research ZymoBIOMICS D4300T User Manual

For Research Use Only Ver. 1.4.1
90
ZymoBIOMICS
™
DNA Miniprep Kit
Catalog Nos. D4300T, D4300, & D4304
Highlights
• Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the
ZymoBIOMICS Microbial Community Standard.
• Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
• Certified Low Bioburden: Boost your detection limit for low abundance microbes.
• Simple Workflow: Simply bead-beat sample, purify via spin-column, and filter to remove PCR inhibitors.
No precipitation or lengthy incubations!
Contents
Product Contents & Specifications ................................. 1
Product Description ..................................................... 2-3
Protocol ........................................................................ 4-5
Appendices
A. Sample Collection ............................................... 6
B. Application Notes ........................................... 7-10
C. Standardize Sample Preparation with
ZymoBIOMICS
™
Microbial Standards ....... 11-12
D. Troubleshooting ........................................... 12-13
Ordering Information ..................................................... 14
INSTRUCTION MANUAL

Zymo Research ZymoBIOMICS D4300T Specifications

General

Brand	Zymo Research
Model	ZymoBIOMICS D4300T
Category	Medical Equipment
Language	English

Summary

Appendix A: Sample Collection

Sample Collection Guidance

Methods for collecting biological samples using DNA/RNA Shield™ for DNA extraction.

Appendix B: Application Notes

Application Notes for Various Samples

Guidance on using the ZymoBIOMICS™ DNA Miniprep Kit for diverse sample types and applications.

Appendix C: Microbial Community Standards

Standardize Sample Preparation

Using mock microbial communities for calibrating DNA extraction and assessing bias.

Bead Beating Device Calibration Protocol

Procedure for calibrating bead beating devices using microbial community standards for unbiased lysis.

ZymoBIOMICS™ Microbial Community DNA Standard

A genomic DNA mixture for assessing library prep, sequencing, and bioinformatics bias.

Appendix D: Troubleshooting

Troubleshooting Common Issues

Common problems and suggested solutions for DNA extraction using the ZymoBIOMICS™ DNA Miniprep Kit.