Zymo Research ZymoPURE Plasmid Miniprep User Manual

ZYMO RESEARCH CORP.
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com
ZymoPURE
™
Plasmid Miniprep Kit
Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending)
Highlights
• Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
• Innovative ZymoPURE
™
technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little
as 25 µl.
Contents
Product Contents ................................................. 1
Product Specifications .......................................... 1
Product Description .............................................. 2
Procedure Overview ............................................. 3
Buffer Preparation ................................................ 4
Protocol ............................................................. 4-6
Troubleshooting ................................................... 7
Ordering Information ............................................ 8
Related Products............................................. 9-10
For Research Use Only Version 1.0.0
INSTRUCTION MANUAL

Zymo Research ZymoPURE Plasmid Miniprep Specifications

General

Format	Spin Column
Sample Type	Bacterial culture
Elution Volume	30-50 µL
Processing	Manual
Storage Conditions	Room Temperature
Downstream Applications	sequencing, transfection
Yield	Up to 20 µg
Technology	Silica-based

Summary

Key Product Specifications

Product Specifications Summary

Summarizes DNA purity, yield, size, recovery volume, required equipment, and processing time for the kit.

Product Description

Procedure Overview

Wash Buffer Preparation

Initial Protocol Steps

Vacuum Protocol

Centrifugation Protocol

Troubleshooting Guide

Ordering Information

DNA Purification Technologies

DNA Purification from PCR & Sources

Methods for purifying DNA from PCR products and other sources using Zymo Research kits.

DNA Recovery from Agarose Gels

Enhance DNA recovery from agarose gels using Zymoclean™ kits.

Plasmid DNA Recovery from Culture

Directly recover transfection-quality plasmid DNA from E. coli cultures.

Additional Zymo Research Products

Heat-Shock Free Competent Cells

Mix & Go! pre-made competent E. coli cells for heat shock-free transformations.

EZ DNA Methylation-Lightning Kits for Bisulfite Conversion