AB Sciex QTRAP 4500 LC/MS/MS system User Manual

AB SCIEX 4500 Series of Instruments
System User Guide
Document Number: RUO-IDV-05-0264-A
Release Date: April 2013

AB Sciex QTRAP 4500 LC/MS/MS system Specifications

General

Brand	AB Sciex
Model	QTRAP 4500 LC/MS/MS system
Category	Laboratory Equipment
Language	English

Summary

Operational Precautions and Limitations

General Safety Information

General safety rules for operating the instrument.

Electrical Precautions

Safety measures for system electrical connections.

Chemical Precautions

Safety procedures for handling chemicals.

Ventilation Precautions

Requirements for proper system ventilation.

Operating Instructions — Hardware

Start Up the System

Step-by-step guide for instrument startup.

Shut Down the System

Instructions for safe instrument shutdown.

Adjust the Integrated Syringe Pump Position

Guide to setting the syringe pump for operation.

Plumb the Diverter Valve

Operating Instructions — Hardware Profiles and Projects

Hardware Profiles

Concept and management of hardware configurations.

Operating Instructions — Tune and Calibrate

Optimize the Instrument

Process for maximizing instrument performance.

Verify or Adjust Performance

Checking and tuning instrument parameters.

Operating Instructions — Acquisition Methods

Create Acquisition Methods

Building methods for data acquisition.

Configure the Syringe Pump

Operating Instructions — Batches

Set Queue Options

Add Sets and Samples to a Batch

Building batches for sample analysis.

Submit a Sample or Set of Samples

Acquire Data

Initiating the data acquisition process.

Set Sample Locations in the Batch Editor

Operating Instructions — Analyze and Process Data

Open Data Files

Loading and accessing acquired data files.

Generate XICs

Operating Instructions — Analyze and Process Quantitative Data

Quantitative Analysis

Process for determining substance concentrations.

Quantitation Methods

Creating and managing analysis methods.

Peak Review and Manual Integration of Peaks

Inspecting and refining peak integrations.

Calibration Curves

Creating and analyzing curves for quantitation.

Sample Statistics

Service and Maintenance Information

Clean the Surfaces

Clean the Front-End

Detailed cleaning of front-end components.

Prepare for Cleaning

Clean the Curtain Plate

Specific instructions for cleaning the curtain plate.

Clean the Front of the Orifice Plate

Specific instructions for cleaning the orifice plate.

Put the System Back into Service

Troubleshooting

System Issues

Common problems and their solutions.