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AB Sciex 4500 Series - User Manual

AB Sciex 4500 Series
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AB SCIEX 4500 Series of Instruments
System User Guide
Document Number: D5030863 A
Release Date: March 2012

Table of Contents

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Summary

Foreword

General Safety Information

Provides critical safety guidelines and precautions for operating the instrument.

Symbols and Conventions

Explains symbols and conventions used throughout the guide for clarity.

Qualified Personnel

Specifies requirements for personnel operating and maintaining the instrument.

Equipment Use and Modification

Outlines guidelines for proper system usage and restrictions on modifications.

Regulatory Compliance

Lists standards and regulations the system complies with in various regions.

Occupational Health and Safety Symbols

Describes common safety symbols found in laboratory environments.

Mains Supply

Details electrical requirements and connection guidelines for the instrument.

Instrument Disposal (Waste Electrical and Electronic Equipment)

Provides instructions for the proper disposal of electronic waste.

Chapter 2 System Information

Theory of Operation

Explains the fundamental principles of mass spectrometry and system operation.

Data Handling

Describes how the system software manages and displays acquired data.

System Overview

Provides a general description of the instrument's front view and main components.

Instrument Panel Symbols

Details the meaning of the instrument's status indicator LEDs.

Turn on the System

Step-by-step guide for powering on the instrument and associated components.

Shut Down the System

Step-by-step guide for safely powering off the instrument and its components.

Configure the Integrated Syringe Pump

Instructions for setting up and configuring the integrated syringe pump.

Configure the Diverter Valve

Procedures for configuring the diverter valve for different operational modes.

Instrument Safe Fluids

Lists fluids that can be safely used with the instrument.

Chapter 3 Hardware Profiles and Projects

Hardware Profiles

Explains the concept of hardware profiles and their role in software configuration.

Create a Hardware Profile

Step-by-step guide for creating a new hardware profile for instrument configuration.

Add Devices to a Hardware Profile

Instructions on how to add and configure specific devices within a hardware profile.

Troubleshooting Hardware Profile Activation

Provides steps to resolve issues when activating a hardware profile.

Create Projects and Subprojects

Guide on organizing data by creating projects and subprojects.

Back up the API Instrument Folder

Recommends and describes procedures for backing up essential instrument folders.

Chapter 4 Instrument Tuning and Calibrating

Optimize the Instrument

Details the process for verifying and optimizing instrument performance.

Verify or Adjust Performance

Explains how to check and fine-tune the instrument's operational parameters.

Results Summary

Describes the summary report generated after instrument optimization.

Chapter 5 Basic Acquisition Methods

Create Acquisition Methods

Step-by-step guide to building acquisition methods using the editor.

Scan Techniques

Explains different types of mass spectrometry scan techniques available.

Quadrupole-Mode Scan Types

Details specific scan types for quadrupole-based mass spectrometry.

LIT-Mode Scan Types

Describes scan types utilizing the Linear Ion Trap (LIT) technology.

Parameters

Lists and explains key instrument parameters used in acquisition methods.

Chapter 6 Batches

Create and Submit a Batch

Guides users on creating and submitting batches for sample analysis.

Acquire Data

Instructions on initiating and managing data acquisition runs.

Queue States and Device Status

Explains how to monitor the status of acquisition queues and instrument devices.

Chapter 7 Analyzing and Processing Data

Open Data Files

Procedures for opening and accessing acquired data files.

Chromatograms

Explains different types of chromatograms and their purposes.

Generate XICs

Details methods for extracting ions to create Extracted Ion Chromatograms (XICs).

Data Processing

Overview of common data processing techniques and tools.

Graphs

Information on manipulating and viewing graphical data representations.

Chapter 8 Analyzing and Processing Quantitative Data

Quantitative Analysis

Introduces the principles and purpose of quantitative analysis.

Quantitation Methods

Explains how quantitation methods are created and used for analysis.

Peak Review and Manual Integration of Peaks

Guides on reviewing and manually adjusting peak integration in data.

Calibration Curves

Details the creation and use of calibration curves for quantification.

Appendix A Turbo V Ion Source User Reference

Introduction to the Ion Source

Provides an overview of the Turbo Vâ„¢ Ion Source and its components.

Probes

Describes the available probes (TurboIonSpray®, APCI) and their specifications.

Installation

Step-by-step instructions for installing the ion source and probe.

Optimization

Guidance on optimizing ion source parameters for best performance.

Maintenance

Outlines recommended maintenance procedures for the ion source.

Troubleshooting

Lists common issues and their corrective actions for the ion source.

Appendix B 4500 Series Instrument Requirements and Parameters

Electrical and Gas Requirements

Specifies the electrical and gas supply requirements for the instrument.

Instrument Parameters

Lists key instrument parameters and their typical ranges for various modes.

Appendix C Calibration Ions and Solutions

Tuning Frequency

Details the recommended frequency for instrument calibration and resolution optimization.

Appendix D Cleaning and Maintenance

System Maintenance Tasks

Presents a schedule for routine cleaning and maintenance of the instrument.

Health and Safety Precautions

Lists important health and safety precautions to follow during cleaning.

Front-End Cleaning

Provides instructions for cleaning the instrument's front-end components.

Appendix E Basic System Troubleshooting

System Issues

Lists common system problems and their potential causes and solutions.

AB Sciex 4500 Series Specifications

General IconGeneral
TypeTriple Quadrupole Mass Spectrometer
Mass AnalyzerTriple Quadrupole
Dynamic Range> 5 orders of magnitude
Ion SourceTurbo V Ion Source
Mass Rangem/z 5 - 2000
Q1 Mass Rangem/z 5 - 2000
Q3 Mass Rangem/z 5 - 2000
SoftwareAnalyst Software
PolarityPositive / Negative
Scan ModesMRM, Precursor Ion Scan, Neutral Loss Scan
ResolutionUnit

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