Applikon my-Control - Table of Contents

OPERATOR MANUAL
CONTENTS my-Control for Autoclavable Bioreactor Systems 2 and 3 L
Software Version mE.2.9.X; Document Version 2.9
2
TABLE OF CONTENTS
1 Safety ..........................................................................................................................................................3
1.1 Safety Symbols ..................................................................................................................................3
1.2 Safety Warnings ................................................................................................................................4
2 General........................................................................................................................................................7
2.1 General Introduction ..........................................................................................................................7
2.2 The User Interface .............................................................................................................................7
2.3 Graphical Presentation of Buttons ...................................................................................................10
2.4 System Tag / Tab presentation ........................................................................................................11
2.5 Entering Text and Numeric Values .................................................................................................11
3 Assembling the Reactor ............................................................................................................................12
3.1 General ............................................................................................................................................12
3.2 Assembling the Head Plate ..............................................................................................................12
3.2.1 2 Liter Reactor (Dished Bottom) .......................................................................................13
3.2.2 3 Liter Reactor (Dished Bottom) .......................................................................................14
3.2.3 Sensor Holder for the LumiSens dO
2
Sensor .....................................................................15
3.3 (Dis)Assembling the Magnetically Coupled stirrer .........................................................................16
4 pH and dO
2
Sensor Preparation ................................................................................................................17
4.1 General ............................................................................................................................................17
4.1.1 Factory Calibration Data for the LumiSens Sensor Tip .....................................................18
4.1.2 Lifetime of the LumiSens Sensor Tip ................................................................................18
4.2 pH Sensor Calibration Routine ........................................................................................................19
5 Dose Monitors Initialization .....................................................................................................................21
5.1 General ............................................................................................................................................21
5.2 Display Values ................................................................................................................................21
6 Autoclaving...............................................................................................................................................22
6.1 Introduction .....................................................................................................................................22
6.2 Autoclaving Procedure ....................................................................................................................23
6.3 Relation between Sterilization Temperature and Time ....................................................................23
7 Preparing Cultivation ................................................................................................................................24
7.1 Installation .......................................................................................................................................24
7.2 Preparing for Operation ...................................................................................................................25
7.3 Calibrating the dO
2
Sensor ..............................................................................................................26
7.4 Resetting Dose Monitor Values .......................................................................................................27
8 Cultivation ................................................................................................................................................28
8.1 Fixed PID Control Versus Adaptive Control ...................................................................................28
8.2 Inoculation .......................................................................................................................................28
8.2.1 Tube Welding ....................................................................................................................29
8.3 Starting the Process Timer ...............................................................................................................29
8.4 Sampling .........................................................................................................................................30
8.5 Decontamination and Harvesting ....................................................................................................30
9 Cleaning ....................................................................................................................................................31
9.1 Cleaning procedure..........................................................................................................................31
9.2 Cleaning the porous sparger ............................................................................................................33