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General| Brand | Applikon |
|---|---|
| Model | ez-Control |
| Category | Laboratory Equipment |
| Language | English |
Explains symbols used in the manual for warnings and information.
Details general safety precautions and warnings related to equipment operation and potential hazards.
Introduces the Applikon In Situ sterilizable Bioreactor Systems and their main components.
Presents the ez-Control system combined with the Pilot System 70L, including a diagram.
Describes the Synoptic and Bar Graph screens displayed after system initialization.
Lists and describes various icons used on the ez-Control interface for navigation and status indication.
Explains the numeric and alphanumeric keypads for data entry, calibration, and password protection.
Details the Bar Graph screen for parameter overview and Trend View screens for historical data visualization.
Explains how to change set-points, noting that Operator authorization is required.
Describes how to edit actuator assignments and rename actuators according to specific demands.
Lists other relevant manuals for the ez-Control system, such as Hardware and PID Control manuals.
Covers initial procedures like unpacking, cleaning, and assembly before preparing for a new process.
Outlines the essential steps for preparing the bioreactor for a new cultivation or fermentation process.
Details the procedure for calibrating the pH sensor, including 1-point and 2-point calibration methods.
Explains the calibration of the DO sensor after sterilization and its potential maintenance needs.
Provides instructions for mounting level sensors and general notes on other sensors like temperature and pressure.
Covers preparation steps for push-valves, tubing, bottles, and other external connections to the reactor.
Lists necessary verifications for steam, cooling water, and gas supplies before proceeding to pressure testing and sterilization.
Describes the manual procedure for performing a pressure hold test to ensure the reactor is airtight.
Outlines the discrete steps involved in the sterilization routine and the operator's required actions.
Presents the specific settings and durations for both empty and full sterilization routines.
Details the activity matrix for various actuators during different stages of empty and full sterilization.
Guides the operator through the step-by-step execution of the sterilization process from start to finish.
Details the steps required to prepare the bioreactor for cultivation after the sterilization process is complete.
Covers height adjustment of the level sensor and calibration procedures for the DO sensor.
Explains how to connect acid and base fluids using the contained addition assembly and push valves for aseptic operation.
Instructs on activating all control loops in the ez-Control system to achieve optimal process conditions for cultivation.
Details the aseptic "Tube Welding" technique for adding inoculum to initiate the cultivation process.
Explains the function and operation of the bottom drain valve for taking samples and harvesting the system.
Provides procedures for draining the reactor and cleaning pH and DO sensors after cultivation.
Outlines two cleaning steps for the bioreactor using specific solutions and temperatures for thorough cleaning.
Details disassembly and cleaning of tubing and auxiliaries using the same cleaning solutions as for the reactor.
Refers users to Maintenance Data Sheets from Applikon-Biotechnology for detailed maintenance instructions.
Describes how the system detects power failures, the resulting messages, and options for restart or halt.
Explains the immediate effect of the Emergency Stop button and the procedures for removing the cause and restarting the system.
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