Applikon ez-Control - Default Chapter; Table of Contents

OPERATOR MANUAL
CONTENTS ez-Control for In Situ sterilizable Bioreactors
Project 310420; PS70 IST (AU)
2
TABLE OF CONTENTS
1 GENERAL SAFETY .................................................................................................................................4
1.1 Safety Symbols ..................................................................................................................................4
1.2 Safety Warnings ................................................................................................................................5
2 GENERAL .................................................................................................................................................7
2.1 Introduction .......................................................................................................................................7
2.2 System Overview ...............................................................................................................................7
2.3 Home Screen .....................................................................................................................................8
2.4 Icons ................................................................................................................................................10
2.5 Keypad and Keyboard .....................................................................................................................11
2.6 Bar Graph and Trend View Screens ................................................................................................12
2.7 Set-Points ........................................................................................................................................13
2.8 Configurable Outputs ......................................................................................................................14
2.9 Related Manuals ..............................................................................................................................14
3 GENERAL PREPARATIONS .................................................................................................................15
3.1 General ............................................................................................................................................15
3.2 Preparing for a New Process ...........................................................................................................15
3.2.1 Calibrate the pH Sensor .....................................................................................................15
3.2.2 Check the DO Sensor ........................................................................................................16
3.2.3 Other Sensors .....................................................................................................................16
3.2.4 Other Hardware .................................................................................................................16
3.2.5 Prepare Supplies ................................................................................................................17
4 PRESSURE TEST AND STERILIZATION ............................................................................................18
4.1 Pressure Hold Test...........................................................................................................................18
4.2 Sterilization Routine ........................................................................................................................18
4.3 Sterilization Settings ........................................................................................................................26
4.4 Actuator Activity Matrix .................................................................................................................27
4.5 Execution of the Sterilization Routine .............................................................................................30
4.5.1 Start ...................................................................................................................................30
4.5.2 Initialization .......................................................................................................................30
4.5.3 Pressure Hold Test .............................................................................................................30
4.5.4 Phase 1 ...............................................................................................................................31
4.5.5 Preheating ..........................................................................................................................31
4.5.6 Phase 2 ...............................................................................................................................31
4.5.7 Sterilization ........................................................................................................................32
4.5.8 Natural Cooling .................................................................................................................32
4.5.9 Cooling 2 ...........................................................................................................................33
4.5.10 Cooling 3 ...........................................................................................................................33
4.5.11 End ....................................................................................................................................33
5 CULTIVATION .......................................................................................................................................34
5.1 Preparing for Cultivation .................................................................................................................34
5.1.1 Prepare the Sensors ............................................................................................................34
5.1.2 Connect Addition Fluids ....................................................................................................36
5.1.3 Activate the Control Loops ................................................................................................37
5.2 Start Cultivation by adding Inoculum ..............................................................................................38
5.3 Drain Valve .....................................................................................................................................39