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MANUAL
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AUTOMATIC &
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ATER RESISTANT
5. Verifying Calibration and Calibration Certification
2. Precautions 4. Automatic Temperature Compensation (ATC)
With each sample, the refractive index varies depending on the temperature. The position of the boundary
line, seen through the eyepiece, will deviate based on the temperature at the time of measurement.
With a non-ATC Hand-held Refractometer, manual calculation for temperature correction is needed. For
example, at 10℃ intervals, a variation of 0.6 to 0.8% can be seen for a standard sugar solution at
10%.
The MASTER-Pα utilizes a built in Automatic Temperature Compensation feature so that the instrument's
internal scale will shift automatically when the ambient temperature changes. This feature eliminates
the need for temperature compensation charts. The refractometer and the sample should be at the
same ambient temperature to ensure that the ATC feature is working correctly.
To measure a heated or refrigerated sample, allow the sample to conform to the ambient temperature
before taking measurements. Waiting 1 to 2 minutes after putting the sample onto the prism will ensure
more accurate readings.
When the measurement value falls outside of the expected range, verify calibration according to section 6.
Calibration.
［1］ Verifying Calibration
Periodic maintenance/service and calibration of your refractometer is recommended. The frequency at
which calibration is performed will depend on each company’s Standard Operating Procedures.
①Confirm that the prism is clean and free of scratches.
②Measure purified water or a sucrose solution. Check that
the boundary line is parallel to the memory lines.
③Verify that the measurement value is within ±0.2％ of the
expected value of the purified water (0.0%) or sucrose
solution.
6. Calibration
If the measurement value is incorrect after verifying calibration
(section 5.) adjust the scale at an ambient temperature of 20℃.
Put purified water on the prism, then view the scale through the
eye piece. To adjust the scale during calibration, turn the scale
adjustment screw located on the underside as shown in the figure
to the right.
［2］ Calibration Certification
Based on ISO quality management system, Calibration Certificates can be ordered for any ATAGO
refractometer. HACCP or GMP certification can also be provided at additional cost. Please contact an
ATAGO distributor for more information.
*Measure at an ambient temperature of 20℃.
1 2 3
4 5
××
3. Measurement
Close the daylight plate gently.
Put one or two drops of sample
on the prism.
The sample must spread evenly
over the prism surface.
Air bubbles should be eliminated.
View the scale through the
eyepiece. To focus, turn eyepiece in
either direction until clear. Use the
number printed on the side of the
eyepiece as a reference for the
position of the eyepiece when it is in
focus.
Read the measurement value
where the boundary line intersects
the scale.
(MASTER-53Pα pictured above.)
Wipe the sample off with a wet tissue
or wash the prism with water.
After washing the prism, wipe off any
remaining water with a dry soft tissue.
6
*Hold the refractometer between your fingers.
Do not wrap your hand around the grip.
RE-110010
RE-110020
RE-110030
RE-110040
RE-110050
Part No.
Part Name
Sucrose Solution 10.0％(g/100g)
Sucrose Solution 20.0％(g/100g)
Sucrose Solution 30.0％(g/100g)
Sucrose Solution 40.0％(g/100g)
Sucrose Solution 50.0％(g/100g)
The MASTER-H series is the heat-resistant model of the MASTER series. They are equipped with corrosion-resistant tempered
glass prism and designed to withstand high-temperature samples. The conventional H-50, H-80, and H-93 refractometers are
recommended for measuring jams during the cooking process.
*Although the unit is rated IP65 dust-tight and
resistant against water jets, it may be
damaged from moisture penetration if left wet
for extended periods of time.
*MASTER-53Pα scale uses 0.2% increments
on the left, and 0.5% increments on the right.
●Do not submerge the unit underwater for a
prolonged period of time.
●
Do not submerge the unit in a hot liquid,
such as a soup simmering in a pot.
●When measuring a hot sample, the sample
should be allowed to cool down to room
temperature before being placed on the prism.
By following this procedure the integrity of
the prism will not deteriorate as quickly if
used to measure hot samples continually.
●Keep the unit away from any heat source,
such as a cook stove.
●Use water at ambient temperature (about
20℃) to clean the prism area after measur-
ing a high-temperature solution. Do not
use cold water (below 10℃).
●The refractometer is a precise and sensitive
optical instrument. Do not drop or subject to
strong shock or excessive force.
●
Store the unit in its original storage case in a
dry, room temperature (0-40 ℃) environment
away from direct sunlight.
●
When measuring a murky or dark solution,
the boundry line may be difficult to make
out or completely invisible. Hold the unit up
to stronger light, such as direct sunlight or
a light source for microscopes.
●The prism and daylight plate should be
completely clean for each measurement.
Oil-based solutions may leave a film-like
residue. Clean the prism and daylight plate
with alcohol or mild detergent diluted with
water.
10℃
10℃
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