II
-
PREPARING
THE
STERILGARD®II
CABINET
FOR
USE
Checking
the
Cabinet
On
Arrival
Upon
receipt
of
your
new
SteriiGARDoll
cabinet,
first
inspect
the
exterior
of
the
crate
and
skid.
If
there
is
any
broken
glass
or
other
visible
damage,
that
fact
should
be
noted
on
the
receiving
slip
and
immediately
reported
to
the
delivering
carrier.
Now
remove
the
crate
and
inspect
the
tmit
itself.
The
top
cover
of
the
crate
should
be
taken
off first,
then
the
boards
from
front
and
back.
Bend
both
ends
of
the
crate
outward
away
from
the
sides
of
the
unit
and
remove
front
or
rear
blocking
from
the
skid.
Remove
the
cabinet
from
the
skid
with
a
fork
lift
or
other
available
equipment.
If
any
concealed
damage
is
found
it
should
be
reported
to
the
delivering
carrier,
who
will
want
m1
opportunity
to
inspect
the
damage.
A
claim
for
restihttion
shollld
be
filed
within
15
days.
.
Because
of
the
dwger
of
mishandling
by
trucking
companies,
we
have
removed
certain
parts
of
the
cabinet
and
have
packed
them
separatelv.
These
items
are
listed
on
the
packing
slip
which
accompanies
the
unit.
Please
check
the
packing
slip
carefully
to
be
sure
that
all
items
have
been
located.
The
Uses
of
a
Biological
Safety
Cabinet
The
SterilGARD"II
cabinet
has
been
designed
to
provide
a
work
area
which
protects
the
experiment
from
the
environment,
and
the
enviroruncnt
frorn
the
experi-ment. The
larninnr
flow biological
safety
cabinet
is
designed
for
work
with
Biosafety Levels 1, 2
and
3 (low
to
moderate
risk)
agents
as
listed
in
The
Center
for
Disease
Control's
~~Biosafety
in
lviicTobiological
and
Biomedical
Laboratories",
U.S.
Department
of
Health
and
Hllman
Services, Public I-Iealth
Service,
Centers
for
Disease
Control
and
National
Institutes
of
Health,
L.S.
Govenunent
Printing
Office,
Washington,
D.C. 20402.
HHS
publication
nurnber
(CDC! 34.-3395.
Biosafety
level
4
or
extremely
f'Ligh
risk
agents
sl1ould neve·r
be
used
in
ul-lls
cabinet. Please
5
consult
your
safety
professional
for a
proper
risk
assessment.
C4.UTIONS
*
T1w
use
of
any
hazardous
material
in the
cabinet requires
that
it
be
monitored
by
an
industrial
hygienist,
safety
officer or
other
qualified i>u/ividual.
* Explosive or flammable su/Jstances
should
never be used in the cabinet
unless
a quali-
fied
safety
professional
has
evaluated
the
risk.
*
lf
hazardous biological
work
is
to
be
per-
formed,
apply
the app1·opriate
biohazard
de-
cal which
is enclosed. This is in accord
with
OSHA
regulations, volume 39,
11umber
125,
PadiL
*
If
chentical, 1·adialogical
or
other
n.on.micro-
bialogical hazards are present,
be
sure
to
employ
appropriate protective measures in
addition
to
formaldehyde decontamination.
Call upol! a
suitably
trailred
individual
to
monitor
the operation.
Location
Within
the
Laboratory
The
ideal
location for
any
laminar
flow
biological safety cabinet is
in
a
dead-end
comer
of
the
laboratory
away
from
personnel
traffic,
vents,
doors,
windows
and any
other
sources
of
dismptive
air
currents.
Published
research
from
The
Baker
Companv
(Rake, B.W.
"Influence
of
Crossdrafts"
on
the
Perfonnance
of
a Biological
Safety Cabinet. Appl. Microbiology
36:278-283,
1978.)
and
w1published tests
performed
at
the
National
Cancer
Institute
show
that
i.f
a
draft
or
other
disruptive
air
current
were
to
exceed
the
intake
velocity
of
the
cabinet,
then
contarrlination
can e-nter
the
vvork
area
or
escape
frorn
it.
Proper
placement
within
the
is
essential.
If
the
cabinet
exhausts
Lts
air
into
the
Iaborrltonr
ins:tead of
venting
to the OL.ltsidef
it
is
imoortant
that
there
be
adequate
space
between
th~
top
of
t._he
cabinet
and
the
ceiling. A soJjd ceiling
located
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