Beckman Coulter VersaLyse Lysing Solution Ready-for-use User Manual

B59380–AD
MADEINFrance
VersaLyse
LysingSolution
.
1mL/test,A09777
100tests;100mL
TABLEOFCONTENTS
English........................................................2
Français(FR).....................................................7
Deutsch(DE)....................................................13
Italiano(IT).....................................................19
Español(ES).....................................................25
PortuguêsPortugal(PT-PT)..............................................31
Dansk(DA).....................................................37
Svenska(SV)....................................................42
Norsk(NO).....................................................47
Ελληνικά(EL)....................................................52
Lietuviškai(LT)....................................................58
Magyar(HU).....................................................63
Polski(PL)......................................................68
Čeština(CZ).....................................................74
Slovenčina(SK)...................................................80
Türkçe(TR).....................................................85
Русский(RU)....................................................90
eestikeel(ET)....................................................96
Hrvatski(HR).....................................................101
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Română(RO)....................................................112
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Latviešu(LV).....................................................122
Українська(UK)...................................................127
APPENDIX.....................................................133
REFERENCES....................................................134
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Beckman Coulter VersaLyse Lysing Solution Ready-for-use Specifications

General

Product Type	Lysing Solution
Brand	Beckman Coulter
Form	Liquid
Storage Temperature	2-8°C
Stability	Stable until expiration date when stored as directed
Intended Use	For use in lysing red blood cells in whole blood samples for flow cytometry analysis
Compatibility	Beckman Coulter Flow Cytometers
Hazard Statements	H319: Causes serious eye irritation
Precautionary Statements	P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Summary

INTENDED USE

PRINCIPLE

WARNING AND PRECAUTIONS

GHS HAZARD CLASSIFICATION

STORAGE AND STABILITY

SAMPLES

WASHED SAMPLES

Details procedures for handling washed samples to ensure optimal performance of VersaLyse.

PREPARATION OF SAMPLES

Guides on adjusting leukocyte and erythrocyte concentrations for optimal sample preparation before lysis.

EVIDENCE OF DETERIORATION

MATERIALS NEEDED BUT NOT SUPPLIED WITH KIT

PROCEDURE with VERSALYSE REAGENT

A - Procedure without concomitant fixation

Details the steps for lysing red blood cells without using a fixative during the incubation period.

B - Procedure with concomitant fixation

Outlines the protocol for lysing red blood cells while using a fixative concurrently.

ANTICOAGULANT

ANALYSIS

PERFORMANCE

INTRA-LABORATORY REPRODUCIBILITY

Summarizes intra-laboratory reproducibility results for CD3+ lymphocytes using VersaLyse with concomitant fixation.

LIMITATIONS

TRADEMARKS

Symbols Key

APPENDIX

EXAMPLES

Presents biparametric graphs illustrating normal whole blood samples analyzed using VersaLyse procedures.

REFERENCES