User's Manual • 6-2
Problem Possible Cause Solution
Fluorescent image has
high background
Image was taken without being
removed from glass plate
Remove plate from sample.
Thin polyacrylamide samples
can be transferred to exposed
x-ray film and imaged using
UV-epi mode
660 NM filter is not in place Install 660 NM filter onto lens
Wrong application selected Verify / reselect correct
application
Light leak Check for light leaks, ensure
Camera Module tightly
connected to
High fluorescence agarose
used
Use low fluorescence agarose
Auto-fluorescence from sample Remove sample and acquire a
control image
Destain (if appropriate) was
insufficient
Increase destain
Obstruction in filter wheel
housing
Check for and remove
obstruction
Custom lens is causing
obstruction
Contact Bio-Rad for
assistance
Bad filter wheel cable or
connection
Reconnect / replace cable
Filter wheel does not
turn
Poorly seated lamps Turn the lamps in the sockets
to make sure lamps are
seated properly in their
sockets
Bad lamps Replace lamps
Door interlock not working Contact Bio-Rad
Epi lamps are not turning
on
Lamp intensity has decreased
with age
Replace lamps
Incorrect f-stop setting Open aperture and reacquire
the image
Integration time to
acquire image has
increased
Incorrect application selected Verify application is correct for
sample
Sample may have degraded Some Chemi samples have a
short lifetime, make sure the
sample is hot and fresh
Incorrect lens/filter used Use the 50 mm high NA lens
for best chemi results and use
no filter Position 5 or higher
Sample on platen with chemi
tray installed
Place sample on chemi tray or
remove tray from sample
chamber
Incorrect f-stop setting Adjust f-stop to a lower value,
f/1.4 recommended.
The 660IR cutoff filter was not
removed from the lens
Remove 660 cutoff filter
Insufficient integration time Integrate sample for a longer
time
Poor
chemiluminescence
sensitivity
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